温度周期性波动对凡纳滨对虾稚虾蜕壳和蜕壳激素含量的影响  

EFFECTS OF TEMPERATURE FLUCTUATION ON THE HEMOLYMPH 20-HYDROXYECDYSONE CONCENTRATION IN JUVENILE LITOPENAEUS VANNAMEI

作　　者：郭彪[1,2] 王芳[1] 董双林[1] 

机构地区：[1]中国海洋大学教育部海水养殖重点实验,山东青岛266003 [2]河北农业大学海洋学院,河北秦皇岛066000

出　　处：《海洋湖沼通报》2011年第1期43-49,共7页Transactions of Oceanology and Limnology

基　　金：国家自然科学基金项目(30571441);国家科技支撑计划项目(2006BAD09A07);国家"973"计划项目(2009CB118706)

摘　　要：采用实验生态学方法,以温度25℃为对照,研究了四种温度波动幅度(25±1、25±2、25±3、25±4℃)对凡纳滨对虾(Litopenaeus vannamei)稚虾蜕壳率和蜕壳激素滴度的影响。主要实验结果如下:(1)25±3℃和25±4℃组对虾的蜕壳率显著高于其他各处理组(P<0.05)。(2)5种温度处理下,对虾血淋巴中蜕壳激素含量在一个蜕壳周期内的变化呈相似的规律:蜕壳间期,对虾血淋巴中蜕壳激素含量逐步增大,至蜕壳前期时,蜕壳激素的含量增至最高值;对虾蜕壳后,血淋巴中蜕壳激素含量急剧下降到一个较低的水平。(3)不同蜕壳时期的对虾在5种温度处理下,其血淋巴中蜕壳激素含量存在显著性差异;蜕壳前期时,25±3℃组对虾蜕壳间期蜕壳激素含量最高,显著高于其他各处理组(P<0.05)。实验结果初步表明,25±3℃变温处理下,蜕壳前期对虾蜕壳激素含量的增加可能是其蜕壳生长的主要原因。Experiment was conducted to examine the effects of temperature fluctuation on the hemolymph 20-HE concentration in juvenile Litopenaeus vannamei.One constant temperature（25 ℃） and four cyclical temperature change regimes（25℃±1℃,25℃±2℃,25℃±3℃,and 25℃±4℃） were conducted.The main results were as follows：（1） the molting rate of shrimps in 25℃±3℃,and 25℃±4℃ treatment were significantly higher than those in other treatments.（2） The hemolymph 20-HE concentration of shrimp kept low level and increased gradually in intermolt period,and increased rapidly and reached peak value in premolt period,then suddenly decreased to the lowest level in postmolt period.（3） The hemolymph 20-HE concentrations of shrimp in different molting period had significantly difference in five temperature treatments;during the pre-molt stage,the hemolymph 20-HE concentration of shrimps in 25±3℃ treatment was significantly higher than those in the other treatments（P0.05）.The results suggested that the temperature fluctuation with 3℃ fluctuating amplitude could promote the molting rate through increasing hemolymph 20-HE concentration at the pre-molt stage.

关 键 词：变温 凡纳滨对虾 蜕壳 蜕壳激素 

分 类 号：S917[农业科学—水产科学]