三种方法评价敌匹硫磷、残杀威与双酚A对小鼠RAW264.7细胞增殖的联合效应  

Study of three methods on joint toxicity of diazinon,propoxur and bisphenol A on proliferation of mouse RAW264.7 cell

作　　者：谭小华[1] 杨杏芬[1] 黄琼[1] 李宁[2] 郝卫东[3] 黄俊明[1] 

机构地区：[1]广东省疾病预防控制中心,广州510300 [2]中国疾病预防控制中心营养与食品安全所 [3]北京大学公共卫生学院

出　　处：《卫生研究》2011年第2期191-195,共5页Journal of Hygiene Research

基　　金：国家科技支撑计划项目(No.2006BAK02A02)

摘　　要：目的探讨农药敌匹硫磷和残杀威以及环境内分泌干扰物双酚A对小鼠RAW264.7细胞增殖活性的联合毒作用类型。方法采用噻唑蓝(MTT)比色法检测细胞活性。通过加权机率单位法计算敌匹硫磷、残杀威与双酚A以及它们的混合物(受试物按等毒性比混合)各自的半数生长抑制浓度(IC50)及其95%CI。采用Marking相加指数法、等效应线图解法和Logistic回归分析法判断敌匹硫磷与双酚A、残杀威与双酚A的联合细胞毒作用类型。结果染毒24h后,敌匹硫磷、残杀威与双酚A对RAW264.7细胞的IC50及其95%CI分别为194.1μg/ml(173.4～217.4μg/ml)、448.4mg/L(358.2～573.2μg/ml)和37.5μg/ml(35.3～39.9μg/ml),而敌匹硫磷与双酚A混合物、残杀威与双酚A混合物的IC50及其95%CI分别为168.8μg/ml(160.1～178.2μg/ml)、253.4μg/ml(236.0～273.0μg/ml)。联合毒性评价结果显示,3种评价方法均将敌匹硫磷与双酚A联合作用类型判断为拮抗作用,而将残杀威与双酚A联合作用类型判断为相加作用。结论敌匹硫磷与双酚A对RAW264.7细胞增殖活性的联合毒作用类型为拮抗作用,而残杀威与双酚A联合毒作用类型为相加作用。Objective To investigate possible joint toxic effects of diazinon,propoxur and bisphenol A（BPA） on proliferation of RAW264.7 cells in vitro.Methods Cytotoxicity was assessed by MTT assay.The median inhibiting concentration values（IC50） and 95% confidence interval（CI） of diazinon,propoxur and BPA individually and in mixture（mixed according to ratio of IC50） were established by weighted probit method.The types of toxic interaction of diazinon BPA and propoxur BPA were assessed by three methods commonly used for binary mixtures,which were Additional Index Method,Equivalent Effect Curve Method and Logistic Regression Method.Results After 24-hour expoxure,the IC50 and 95% CI of diazinon,propoxur and BPA to RAW264.7 cells were 194.1μg/ml（173.4μg/ml-217.4μg/ml）,448.4mg/L（358.2μg/ml-573.2μg/ml）,and 37.5μg/ml（35.3μg/ml-39.9μg/ml）,respectively.Those of mixtures of diazinon BPA and propoxur BPA were 168.8μg/ml（160.1μg/ml-178.2μg/ml） and 253.4μg/ml（236.0-273.0μg/ml）.In the interaction assessment,three methods all demonstrated an antagonistic action of diazinon BPA and an addition action of propoxur BPA.Conclusion It is concluded that the interaction of diazinon BPA on proliferation of RAW264.7 cells is antagonistic,and that of propoxur BPA is additive.

关 键 词：敌匹硫磷 残杀威 双酚A 联合毒性 RAW264.7细胞 

分 类 号：R994.6[医药卫生—毒理学] R139.3[医药卫生—药学]