UPLC-ESI-Q-TOF-MS在分析山茱萸化学成分中的应用  

Application of UPLC-ESI-Q-TOF-MS on Chemical Constitutents of Cornus officinalis

作　　者：刘颖坤[1] 喻卫武[1] 白岩[1] 

机构地区：[1]浙江农林大学林业与生物技术学院亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《中国现代应用药学》2011年第3期226-230,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省森林培育重中之重学科开放基金(200511);浙江农林大学预研项目(2009FK44)

摘　　要：目的采用超高效液相色谱电喷雾四级杆飞行时间串联质谱(UPLC-ESI-Q-TOF-MS)对山茱萸中水溶性成分以及醇溶性成分进行分析和鉴别。方法用ACQUITY UPLC HSS T3 C18柱(2.1 mm×100 mm,1.8μm),以0.2%甲酸水溶液-0.2%甲酸甲醇溶液为流动相梯度洗脱;使用ESI离子源,分别在正离子和负离子模式下采集数据。结果推断出山茱萸水溶性成分5种:没食子酸,莫罗忍冬苷,马钱素,山茱萸新苷Ⅰ,当药苷;醇溶性成分5种:7-脱氢马钱素,山茱萸新苷Ⅱ,槲皮素,齐墩果酸,熊果酸。结论经过超高效液相色谱的分离,借助Q-TOF-MS测定得到的精确分子质量及正负离子信息可以鉴定山茱萸中水溶性成分和醇溶性成分,为其成分鉴定提供了一种准确有效的方法。OBJECTIVE To analyze and identify water-soluble composition and alcohol-soluble composition in Comus officinalis. METHODS These constituents were determined by UPLC-ESI-Q-TOF-MS. The chromatographic separation was performed on ACQUITY UPLC HSS T3 C18 （2.1 mm×100 mm, 1.8 um ） with a gradient elution of water containing 0.2% formic acid-methanol containing 0.2% formic acid. The mass spectrometer equipped with electrospray ionization source was used as a detector. And data was collected under the positive and negative ion modes. RESULTS Five water-soluble constituents were identified as gallic acid, morroniside, loganin, cornuside I , sweroside. And five alcohol-soluble constituents were identified as 7-dehydrologanin, cornuside II, quercetin, oleanolic acid, ursolic acid. CONCLUSION In this study, water-soluble and alcohol-soluble constitutents in Comus officinalis were separated by UPLC, and identified through the information of positive ion and negative ion and accurate MW（molecular weight） which were determined by UPLC-ESI-Q-TOF-MS. It is an accurate and effective method which can be applied for the constituent identification of Comus officinalis.

关 键 词：山茱萸 水溶性成分 醇溶性成分 超高效液相色谱电喷雾四极杆飞行时间串联质谱 碰撞诱导解离 

分 类 号：R284.1[医药卫生—中药学]