双波长切换HPLC同时测定桂枝甘草汤中4种成分的含量  

Simultaneous Determination of Four Components in Guizhi-Gancao Decoction by HPLC with Double-wavelength Switch

作　　者：陈新阳[1] 毕开顺[1] 易丽昕[1] 黄文芳[1] 陈晓辉[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中国现代应用药学》2011年第3期265-267,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立桂枝甘草汤中4种成分同时含量测定的方法。方法采用高效液相色谱法，KromasilC18柱（4．6mm×250mm，5um），以乙腈-0．1％甲酸水溶液为流动相进行梯度洗脱，流速：1．0mL·min-1，双波长切换时间序列采样：0～29．5 min为276nm；29．5～32．0min为370nm。结果甘草苷、肉桂酸、桂皮醛、异甘草素分别在10．0～160，2．50～40．0，16．0～256，0．050～0．8ug·mL-1内，线性关系良好。结论该方法准确、简便、专属性好，可用于桂枝甘草汤中甘草苷、肉桂酸、桂皮醛和异甘草素4种成分的含量测定，为桂枝甘草汤的质量控制提供依据。OBJECTIVE To develop an HPLC method for determination of liquiritin, cinnamic acid, cinnamaldehyde and isoliquiritigenin in Guizhi-Gancao decoction with gradient elution and double-wavelength switch. METHODS The analysis was carried out on a Kromasil C18 （4.6 mm×250 mm, 5 pro） column. The mobile phase was composed of acetonitrile and 0.1% formic acid aqueous with gradient elution and the flow rate was set at 1.0 mL·min-1. The detection wavelengths were set at 276 nm for liquiritin, cinnamic acid and cinnamaldehyde （0-29.5 min） and 370 nm for isoliquiritigenin （29.5-32.0 min）. The column temperature was 35 ℃. RESULTS The linear ranges of liquiritin, cinnamic acid, cinnamaldehyde and isoliquiritigenin were 10.0-160, 2.50-40.0, 16.0-256, 0.050-0.8 ug.mL-1, respectively. CONCLUSION This method can be used to determine the above 4 components with satisfied accuracy and repeatability.

关 键 词：高效液相色谱法 桂枝甘草汤 甘草苷 肉桂酸 桂皮醛 异甘草素 

分 类 号：R917.101[医药卫生—药物分析学]