AtEDR1介导拟南芥对稻瘟菌的抗性(英文)  

AtEDR1 Mediated Resistance to Magnaporthe oryzae in Arabidopsis thaliana

作　　者：郭传宇[1] 郑银英[1] 崔百明[1] 

机构地区：[1]石河子大学农业生物技术重点实验室,石河子大学生命科学院,石河子832003

出　　处：《植物病理学报》2011年第2期188-195,共8页Acta Phytopathologica Sinica

基　　金：National transgenic major projects(2008ZX08005-002)

摘　　要：稻瘟菌(Magnaporthe oryzae)是一种半腐生病原菌,该病原菌可以侵染水稻,产生稻瘟病。对于水稻与稻瘟菌的互作机制,人们已经进行了大量的研究。然而,鉴于水稻基因组相对较大且稻瘟菌生理小种众多,致使研究进展缓慢。本研究通过将稻瘟菌生理小种Y34侵染拟南芥生态型Col-0,发现Y34可以感染Col-0,从而建立Y34-拟南芥互作模型,用以探究Y34与抗白粉突变体edr1的关系。结果显示,edr1比Col-0对于稻瘟菌更敏感。之前有研究表明,降低SA含量的突变体(pad4,sid2,nim1)均可抑制edr1相关表型。接种Y34于edr1与pad4、sid2、nim1形成的双基因突变体发现,所有双突变体的感病性比edr1单突均明显降低。推测EDR1可能在拟南芥抗稻瘟菌方面起一定正调控作用,且其作用依赖于SA途径的相关基因。Magnaporthe oryzae（M.oryzae）,a hemibiotrophic pathogenic fungus,can infect rice（Oryza sativa） and cause rice blast.The mechanisms involved in resistance of rice to blast have been studied extensively.However,it is difficult to investigate interactions between rice and M.oryzae because of the relatively large genome sequence of rice and the diversity of M.oryzae.The strain of M.oryzae,Y34,was found to be able to infect Arabidopsis thaliana Col-0 plants,so M.Oryzae（Y34）—Arabidopsis thaliana pathosystem was established to investigate the role of Arabidopsis thaliana ENHANCED DISEASE RESISTANCE 1（AtEDR1）,which exerts negative regulation on powder mildew resistance by salicylic acid（SA） pathway.The results indicated that edr1 mutant was more susceptible to M.oryzae than wild type Col-0 plants.Previous double mutant analysis revealed that all edr1-associated disease resistance phenotypes were suppressed by mutants that block SA perception（nim1） or reduced SA production（pad4 and sid2）.These double mutants were inoculated with Y34 to examine if they would suppress the susceptibility of edr1 to M.oryzae,and the results showed that compared with edr1,all double mutants exhibited resistance to M.oryzae.These data suggest AtEDR1 may play a positive role in the regulation of resistance to M.oryzae in Arabidopsis,which is required for SA pathway.

关 键 词：edrl 稻瘟菌 抗病性 拟南芥 

分 类 号：S432[农业科学—植物病理学]