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作 者:肖仲久[1,2] 李红玫[1,2] 蒋选利[1,2]
机构地区:[1]贵州大学农学院,贵阳550025 [2]贵州大学贵州省山地农业病虫害重点实验室,贵阳550025
出 处:《植物病理学报》2011年第2期210-214,共5页Acta Phytopathologica Sinica
基 金:supported by a grant from the Ministry of Science and Technology,Finance,Special Action of Richening Farmer and Strengthening County Research Program(205)
摘 要:Identification of tuber rot pathogen on Stachys sieboldii Miq.was carried out with morphological and molecular methods for the first time.Though the observation of morphology and color of the colonies,morphology of macroconidia,microconidia and chlamydospores,it was preliminarily identified as Fusarium oxysporum.The fungus DNA was amplified by PCR with the universal primers of fungal rDNA ITS1 and ITS4,and sequenced.The sequencing results were logged in GenBank to BLAST,and all of the molecular detections and morphologic observations showed that the pathogen was F.oxysporum.Identification of tuber rot pathogen on Stachys sieboldii Miq.was carried out with morphological and molecular methods for the first time.Though the observation of morphology and color of the colonies,morphology of macroconidia,microconidia and chlamydospores,it was preliminarily identified as Fusarium oxysporum.The fungus DNA was amplified by PCR with the universal primers of fungal rDNA ITS1 and ITS4,and sequenced.The sequencing results were logged in GenBank to BLAST,and all of the molecular detections and morphologic observations showed that the pathogen was F.oxysporum.
分 类 号:S436.8[农业科学—农业昆虫与害虫防治]
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