HPLC法测定人工养殖蟒蛇胆汁中牛磺蟒胆酸钠的含量  

Determination of Sodium Tauropythocholate in the Bile of Artificial Cultured Python

作　　者：符秀娟[1] 武文[2] 石书江[2] 陈志维[2] 龙丽娜[1] 谭家明 苏子仁[2] 

机构地区：[1]海南职业技术学院,海口570216 [2]广州中医药大学,广州510006 [3]海南东盛弘蟒业科技股份有限公司,海口571000

出　　处：《中药新药与临床药理》2011年第2期194-196,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：海南省自然科学基金项目(309128);海南省重点科技计划项目(ZDXM20100003)

摘　　要：目的建立测定人工养殖蟒蛇胆汁中牛磺蟒胆酸钠含量的方法。方法采用高效液相色谱法测定其含量。色谱条件：Phenomenex Luna C18色谱柱（250 mm×4.60 mm,5μm）;流动相为乙腈-0.15%磷酸氢二钠（10%磷酸调pH至3.0）,梯度洗脱;流速为1.0 mL.min-1;检测波长203 nm;柱温35℃;进样量10μL。结果牛磺蟒胆酸钠在20.2~322.4μg.mL-1（r=0.998 9）浓度范围内线性关系良好,平均回收率为102.4%（RSD=1.4%,n=6）;8批蟒蛇胆汁中牛磺蟒胆酸钠含量差异较大,平均含量为65.09 mg/g-1。结论本方法准确、快速、灵敏、重复性好,可用于蟒蛇胆汁的质量控制。Objective To establish a method for the determination of sodium tauropythocholate in the bile of artificial cultured python.Methods High performance liquid chromatography（HPLC） was carried out on a Phenomenex Luna C18 column（250 mm × 4.60 mm,5 μm） with the mobile phase consisting of acetonitrile and 0.15 % disodium hydrogen phosphate（adding 10 % phosphoric acid to adjust pH value being 3.0） and by using a linear gradient elution.The flow rate was 1.0 mL·min-1,and UV detection wavelength was set at 203 nm.The column temperature was 35 ℃ and the injection volume was 10 μL.Results The calibration curve of sodium tauropythocholate was linear in the concentration range of 20.2 ~ 322.4 μg·mL-1（r=0.998 9）,and the average recovery rate was 102.4 %（RSD=1.4 %,n=6）.There was great difference of sodium tauropythocholate content in 8 batches of python bile,and the average content was 65.09 mg/g.Conclusion The established method is precise,rapid and accurate,which can be used for quality control of python bile.

关 键 词：蟒蛇 胆汁 牛磺蟒胆酸钠 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]