HPLC法测定银翘柴桂颗粒连翘酯苷的含量  

Determination of Forsythiaside in Yinqiao Chaigui Granula by HPLC

作　　者：王带媚[1] 鲍涵[1] 张军[1] 左俊岭[1] 陈冠林[1] 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中药新药与临床药理》2011年第2期197-199,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家科技部"十一五"支撑计划项目(2006BAI01B05)

摘　　要：目的建立HPLC测定银翘柴桂颗粒连翘酯苷含量的方法。方法采用Kromasil C18柱(250 mm×4.6 mm,5μm)为固定相;以甲醇-0.1%磷酸溶液(34∶66)为流动相;流速:1.0mL.min-1;检测波长:330 nm。结果连翘酯苷在0.345～4.140μg范围内线性关系良好,r=1.000 0;平均加样回收率为101.45%,RSD为1.67%(n=6)。结论本方法简便、准确、可靠,重复性好。Objective To establish a HPLC method for the determination of forsythiaside in Yinqiao Chaigui Granula.Methods Kromasil C18 column（250 mm × 4.6 mm,5 μm） was used.The mobile phase was methanol-0.1 % phosphoric acid aqueous（34 ∶ 66） with a flow rate of 1.0 mL·min-1,and the detection wavelength was 330 nm.Results The linear response ranges was 0.345～4.140 μg for forsythiaside,r=1.000 0.The average recovery was 101.45 %,and RSD was 1.67 %（n=6）.Conclusion The established method is simple,accurate and reliable,and with good reproducibility for the determination of forsythiaside in Yinqiao Chaigui Granula.

关 键 词：银翘柴桂颗粒 连翘酯苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]