白芍药材UPLC特征指纹图谱研究  

UPLC characteristic chromatographic profile of Paeoniae Radix Alba

作　　者：张琦[1] 王振中[2] 萧伟[2] 张靓琦[1] 李清[3] 毕开顺[3] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2011年第6期712-714,共3页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09308-003;2009ZX09504-004)

摘　　要：目的:建立白芍药材的UPLC特征指纹图谱分析方法,为快速评价白芍药材的质量,完善白芍的质量控制方法提供依据。方法:采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相乙腈-0.05%磷酸水溶液,检测波长230 nm。结果:建立了白芍药材的UPLC特征指纹图谱共有模式,标定了15个共有峰,指认了其中5个共有峰,15批白芍药材的相似度为0.891～0.996。结论:该方法快速,可用于评价白芍药材质量。Objective： To develop an UPLC method of determining the characteristic chromatographic profiles of Paeoniae Radix Alba for quality control.Method： The UPLC characteristic chromatographic profiles of fifteen batches of Paeoniae Radix Alba were determined on an HSS T3 Column（2.1 mm×100 mm,1.8 μm） eluted with the mobile phases of water containing 0.05% phosphoric acid and acetonitrile in gradient mode.The detection wavelength was set at 230 nm.Result： The common mode of the UPLC characteristic chromatographic profile was set up.There were 15 common peaks,five of which were identified,and the similar degrees of the fifteen samples to the common mode were between 0.891 and 0.996.Conclusion： The method was time-saving and can be used for the quality control of Paeoniae Radix Alba.

关 键 词：白芍 特征指纹图谱 UPLC 

分 类 号：R284[医药卫生—中药学]