藤黄药材的指纹图谱研究  

Fingerprint analysis of gamboge by HPLC

作　　者：侯文洁[1,2] 陈保来[3,2,4] 萧伟[3,2,4] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]江苏康缘药业股份有限公司,江苏连云港222001 [4]江苏省企业院士工作站,江苏连云港222001

出　　处：《中国中药杂志》2011年第6期775-779,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2008ZX09101-Z-024)

摘　　要：目的:建立藤黄药材的高效液相指纹图谱方法。方法:采用Luna C8(4.6 mm×250 mm,5μm)色谱柱,柱温25℃,以乙腈-0.1%冰乙酸水溶液为流动相梯度洗脱,检测波长362 nm,流速为1.0 mL·min-1。采用国家药典委员会出版的《中药色谱指纹图谱相似度评价》(2004年A版)软件,对11批不同批次的藤黄药材指纹图谱进行相似度计算。结果:各批藤黄药材中有13个共有峰,各峰分离度良好,各批次藤黄药材间共有峰的相对保留时间RSD均<1.0%,药材间相似度均>90%。结论:本方法具有良好的精密度、重复性、稳定性,各共有峰间分离度高,可用于藤黄药材的真伪鉴别和质量综合评价。Objective： To establish the chromatographic fingerprint for the quality control of gamboge.Method： Analysis on a Luna C8（4.6 mm×250 mm,5 μm）column eluted with mobile phases containing acetonitrile and 0.1% glacial acetic acid in water in gradient mode.The flow rate was 1.0 mL·min^-1 and the detection wavelength was at 362 nm.The temperature of column was 25 ℃.And data of 11 batches of gamboge samples from different sources were analysed by ＂similarity evaluation for chromatographic Fingerprint of Traditional Chinese Medicine＂ software.Result： Thirteen common peaks were selected in chromatograms,and all the common peeks were separated effectively.Conclusion： The precision,repeatability,and stability of this method were satisfying.The method developed can be used to identify and evaluate the quality of gamboge.

关 键 词：藤黄 HPLC 指纹图谱 

分 类 号：R284[医药卫生—中药学]