偃麦草ErABF1基因克隆及功能分析  

Cloning and Functional Analysis of ErABF1 Gene from Elytrigria repens

作　　者：高世庆[1] 唐益苗[1] 杨颖[1] 柳珊[1] 陈京瑞[1] 马锦绣[1] 田立平[1] 张风廷[1] 赵昌平[1] 

机构地区：[1]北京农林科学院杂交小麦工程技术研究中心,北京100097

出　　处：《麦类作物学报》2011年第2期194-201,共8页Journal of Triticeae Crops

基　　金：国家抗逆转基因重大专项(2008ZX08002-002;2008ZX08002-003;2008ZX08002-004);北京市科技新星项目(2007B056;2008B035);北京农林科学院院青年基金项目;北京市自然基金项目(5102016)

摘　　要：抗逆相关bZIP(Basic leucine zipper)转录因子家族基因主要参与ABA、干旱、高盐等胁迫应答反应,其过表达能够显著增强植物的抗逆性。本研究从偃麦草(Elytrigria repens L.)中分离到一个抗逆相关ErABF1(E.repens ABA Binding Factor 1)基因,氨基酸序列比对分析发现,该基因与小麦、玉米、拟南芥等bZIP转录因子基因同源性较高,亲缘关系较近;ErABF1基因的表达受到ABA、干旱、高盐、低温的强烈诱导;在2%PEG、200mmol·L-1 NaCl胁迫培养基上初步功能分析表明,ErABF1过表达提高了转基因烟草对干旱、高盐的胁迫耐性。Abiotic stresses such as drought,high-salt and low temperature severely affect the growth and development of plant.It was reported that transcription factors played vital roles in regulating downstream gene expression and improving of adversity-resistance in plant.Stress-related bZIP transcription factors（basic leucine zipper） mainly participated in response to ABA,drought and high-salt stresses and its over-expression could significantly enhance the adversity-resistance of plants.In this study,Elytrigria repens with strong drought and salt tolerance was used to isolate and obtain a bZIP transcription factor gene,which was nominated as ErABF1（E.repens ABA Binding Factor 1）.Sequence analysis of amino acids showed that ErABF1 had higher homology and rather relative relationships with bZIP transcription factors of Arabidopsis,wheat and maize,etc.The expression of ErABF1 was intensively induced by ABA,drought,high-salt and low-temperature.Preliminarily functional analysis on the stress medium containing 2% PEG and 200 mM NaCl showed that ErABF1-overexpression improved the tolerance to drought and high-salt stresses in transgenic tobacco plants.

关 键 词：偃麦草 ErABF1 烟草 胁迫耐性 

分 类 号：S512.9[农业科学—作物学] S330