稠油降解菌的筛选、鉴定与菌群构建  

Screen and identification of heavy oil degrading bacteria and communication construction

作　　者：王海峰[1,2] 李阳[3] 赵丽萍[4] 王增林[2] 张建 包木太[5] 

机构地区：[1]胜利勘察设计研究院有限公司,东营257000 [2]胜利石油管理局,东营257000 [3]中国石油化工股份有限公司,北京100029 [4]滨州学院,滨州256600 [5]中国海洋大学海洋化学理论与工程技术教育部重点实验室,青岛266100

出　　处：《环境工程学报》2011年第4期925-929,共5页Chinese Journal of Environmental Engineering

基　　金：“十一五”国家科技支撑计划(2006BAB04B02);胜利石油管理局博士后项目(GKB10020);山东省环境保护科技计划(2006063);山东省博士基金(BS2010H2020)

摘　　要：分别以烷烃、芳烃、胶质沥青质为唯一碳源,从稠油污染过的土壤里分离、筛选可培养的降解菌,将16株组合构建SL-16稠油降解菌群,通过室内摇瓶实验测得该菌群在最佳条件下对陈庄油田稠油降解率可达68%,其适宜的生长及降解温度为35～45℃,pH值为7.0～9.0,含盐量为4 000～14 000 mg/L,接种量为2%,稠油初始浓度为1 000 mg/L,摇床转速为140 r/min。Alkanes,aromatic,resins and asphaltene were used respectively as the sole carbon source to isolate and screen out the culturable degrading bacteria from heavy oil polluted soil,sixteen of which were used to construct the bacterial community SL-16.Through shaking experiments on the bacteria community,it was found that the degradation rate for the heavy oil from Chenzhuang Oil Field reached 68% under the optimal degradation conditions. The genial tempterature for its growth and degradation was 35～45℃,the pH was 7.0～9.0,the salinity rate was 4 000～14 000 mg/L,the inoculation was 2%,the initial heavy oil concentration was lower than 1 000 mg/L,and the rotating speed was 140 r/min.

关 键 词：稠油 石油烃降解菌 烷烃 芳烃 胶质沥青质 16S RDNA 

分 类 号：X703.1[环境科学与工程—环境工程]