pAD-TGF-β1对骨组织工程种子细胞转归的转基因保护  

Study on protection of pAD-TGF-β1 gene transfection to the fate of seed cells in bone tissue engineering

作　　者：易诚青[1] 曹云[1] 马春辉[1] 滕松松 张国桥[1] 朱金宏[1] 宋旺盛[1] 

机构地区：[1]上海交通大学附属第一人民医院骨科,200080

出　　处：《国际骨科学杂志》2011年第2期127-128,135,共3页International Journal of Orthopaedics

基　　金：国家自然科学基金面上项目(30700853);上海市卫生局青年科研基金项目(044Y18)

摘　　要：目的探讨转化生长因子-β1(TGF-β1)真核表达载体质粒pAD-TGF-β1基因转染对骨组织工程种子细胞骨髓基质干细胞(MSC)体外转归的转基因保护价值。方法采用荧光显微镜下观察转染细胞的绿色荧光蛋白表达,噻唑蓝比色法检测细胞增殖情况,核酸原位末端标记法检测细胞凋亡指数。结果荧光显微镜下证实质粒pAD-TGF-β1确切表达于MSC,并显著提高细胞增殖率,降低细胞凋亡指数。结论通过pAD-TGF-β1转基因修饰种子细胞MSC,可有效标记MSC并明显改善MSC转归。Objective To explore the protection of transforming growth factor-β1（TGF-β1） eukaryotic expression plasmid pAD-TGF-β1 gene transfection to the fate of seed cells in bone tissue engineering.Methods Green fluorescent protein（GFP） expression in bone marrow stromal cells（MSCs） was observed in pAD-TGF-β1 transfected MSCs by fluorescence microscope,and the proliferation potential and the apoptosis index（AI） value of MSCs were detected by methyl thiazolyl tetrazolium（MTT） and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling（TUNED methods.Results pAD-TGF-β1 was expressed in MSCs exactly,demonstrated by fluorescence microscope.Proliferation potential of transfected MSCs was enhanced,while Al value decreased significantly.Conclnsion Transfecting cultured MSCs with pAD-TGF-β1 can not only mark them effectively with GFP,but also promote their survival potential.

关 键 词：转化生长因子-Β1 基因转染 种子细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]