尿液和头发及唾液中尼古丁及其代谢物可替宁的气相色谱-质谱同时测定法  

Simultaneous Determination of Nicotine and Metabolite Cotinine in Urine,Hair and Saliva by Gas Chromatography-Mass Spectrometry

作　　者：何怡[1] 徐东群[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《环境与健康杂志》2011年第2期150-153,共4页Journal of Environment and Health

基　　金：国家"十一五"科技支撑计划(2008BAI56B07)

摘　　要：目的建立利用气相色谱-质谱（GC-MS）法同时测定人体尿液、头发、唾液中尼古丁及其代谢物可替宁的方法。方法将样品（消解完全的头发、尿液、唾液）经NaOH碱化后用三氯甲烷萃取，气相色谱分离，质谱检测采用选择离子扫描，并以二苯胺作为内标物进行定量。尿液中尼古丁和可替宁的浓度用尿肌酐校正。结果该方法的尼古丁和可替宁的检出限为分别为6．5、14．5ng／ml，测定下限分别为22、48ng／ml；尼古丁和可替宁的线性范围分别为0．022—4μg／ml、0．048～4μg／ml。尿液中尼古丁和可替宁的精密度分别为2．02％-2．62％、5．27％-7．24％；头发中尼古丁的精密度为1．09％～2．89％；唾液中可替宁的精密度为4．89％～4．54％。尿液中尼古丁及可替宁的加标回收率分别为82．76％～106．47％、81．87％～99．53％；头发中尼古丁的加标回收率为85．37％。103．91％；唾液中可替宁的加标回收率为80．92％-93．78％。结论该实验方法简单、快速、准确，能满足人群烟草暴露评价的需要。Objective To establish a assay method for simultaneous determination of nicotine and its metabolite cotinine in urine, hair and saliva by gas chromatography-mass spectrometry. Methods The sample （urine, hair and saliva） was alkalized by NaOH, diphenylamine as an internal standard, and nicotine and cotinine were extracted simultaneously with chloroform, and then were determined by gas chromatography-mass spectrometry, the concentration of nicotine and cotinine in urine would be corrected by creatinine. Results The limits of detection of the method were 6.5 ng/ml and 14.5 ng/ml for nicotine and cotinine respectively. The limits of quantification were 22 ng/ml and 48 ng/ml respectively. The line ranges of the method were 0.022-4μg/ml and 0.048-4 μg/ml for nicotine and cotinine respectively. The precisions of the method were 1.98%-2.69% and 4.70%-6.65% for nicotine and cotinine in urine, 0.87%-2.89% for nicotine in hair, 4.89%-4.54% for cotinine in saliva respectively. The recoveries of the spiked samples were 82.40%-106.48% and 81.80%-99.55% for nicotine and cotinine respectively in urine, 85.37%- 103.91% for nicotine in hair, 80.50%-93.00% for cotinine in saliva. Conclusion The method is simple, rapid and accurate, and it is satisfying to evaluate the human tobacco exposure.

关 键 词：色谱法 气相 烟草烟污染 尼古丁 可替宁 尿液 头发 唾液 

分 类 号：O657.7[理学—分析化学]