小儿急性肠梗阻细菌移位及血清IL-10变化的临床意义  

Study on clinical value of bacterial translocation and expression change of IL-10 in children with acute intestinal obstruction

作　　者：刘文跃[1] 王琳[2] 王建峰[3] 

机构地区：[1]山西医科大学儿科系,太原030001 [2]山西省儿童医院,030013 [3]山西医科大学普外科,030001

出　　处：《中国医疗前沿》2011年第4期11-12,共2页China Healthcare Innovation

基　　金：山西省科技攻关项目(编号:20080311064-2)

摘　　要：目的应用PCR技术检测小儿急性肠梗阻细菌移位,探讨血清白细胞介素-10(IL-10)变化及临床意义。方法应用聚合酶链反应(PCR)定性检测小儿急性肠梗阻各组及对照组全血细菌DNA;应用酶联免疫吸附试验(ELISA)检测小儿急性肠梗阻组术前、术后及对照组血清中IL-10浓度。结果肠切除肠吻合手术组16SrRNA、大肠杆菌(BG)阳性率分别为86.4%、72.7%;未切除肠管手术组16SrRNA、BG阳性率分别为64.3%、42.8%;保守治疗组16SrRNA、BG阳性率分别为57.1%、28.5%,正常血清对照组16SrRNA、BG均未检出;肠梗阻各组术前血清IL-10浓度高于对照组(P<0.01);各组间术前血清IL-10浓度比较有差异(P<0.05);肠梗阻各组术前与术后比较有差异(P<0.01)。结论急性肠梗阻患儿发生细菌移位,随病情加重发生细菌移位机会增加,检测IL-10对小儿急性肠梗阻细菌移位的发生机制及判断愈后有着重要意义。Objective Polymerase chain reaction（PCR） was used to detect the bacterial translocation in children with acute intestinal obstruction and to investigate clinical value of interleukin-10（IL-10） change in serum.Methods The presense of bacterial DNA in whole blood were detected by polymerase chain reaction（PCR）;Enzyme-linked immunosorbent assay（ELISA） was used to detect the levelof IL-10 in serum.Result In the bowel resection anastomosis group and without bowel resection group,16SrRNA were positive in 86.4% and 64.3% of the patients respectively,BG were positive in 72.7% and 42.8% respectively;In the conservative treatment group,16SrRNA were positive in57.1% of the patients,BG were positive in 28.5%.Bacteria DNA were not detected in the control group.In the three groups of Intestinal obstruction,level of IL-10 in serum before surgery were all higher than the control group（P0.01）;there were difference within group;level of IL-10 in serum before surgery were different from IL-10 in 7 days after surgery in the three groups of Intestinal obstruction.Conclusion Bacterial translocation occurs in children with acute intestinal obstruction,with aggravation the rate increased;The serum IL-10 level is of great implication to know pathogenesis of bacterial translocation and predict prognosis.

关 键 词：急性肠梗阻 细菌移位 IL-10 

分 类 号：R725.7[医药卫生—儿科]