甲氨蝶呤对CIA大鼠血清软骨寡聚基质蛋白的作用  

The role of MTX on the serum COMP of collagen-induced arthritis rats

作　　者：白洁[1] 王秦[1] 马丽辉[1] 罗静[1] 吕志勤[1] 薛丽巾[1] 国华[1] 李芳[1] 李小峰[1] 

机构地区：[1]山西医科大学第二医院风湿免疫科,太原030001

出　　处：《中国医疗前沿》2011年第4期34-35,共2页China Healthcare Innovation

摘　　要：目的探讨甲氨蝶呤(MTX)对CIA大鼠血清软骨寡聚基质蛋白水平的调节作用。方法选取SD大鼠30只,随机分为3组,其中10只未经行造模,未进行移植治疗为空白对照组;另20只造模成功后为两组:标准对照组及MTX治疗组。MTX治疗组每周予以MTX 5mg/kg灌胃×3次,空白组予以同等剂量的生理盐水,每周断尾取血1.5ml。留取血清比较三组的软骨寡聚基质蛋白(COMP)数值。结果造模组血清COMP水平明显高于标准对照组;血清COMP水平与大鼠关节炎指数明显相关;MTX治疗组血清COMP水平明显高于标准对照组,比较有显著性差异(P<0.05)。结论血清软骨寡聚基质蛋白水平在急性炎症关节炎中明显升高,与炎症指数相关,MTX治疗组可以下调炎症关节炎血清软骨寡聚基质蛋白水平,从而延缓大鼠软骨的破坏进程。Objective To investigate the methotrexate（MTX） on the serum cartilage oligomeric matrix protein（COMP） of collagen-induced arthritis rats.Methods 30 SD rats were randomly selected and divided into 3 groups.10 rats as the normal group,without modeling and transplantation;another 20 rats as the model group were successful modeled and then randomly divided into control group and MTX group.Every week,MTX group were injected MTX through Intragastric administration for 3 times.The contral group was given the same dose of physiological saline.The serum COMP in between of three groups were detected weekly.Results The serum COMP levels of the model group were significantly higher than the one of the normal group;The serum COMP levels was significantly related to the arthritis index of rats;The serum COMP levels of translation group were significantly higher than the control groups,there were significantly difference（P0.05）.Conclusion Serum levels of cartilage oligomeric matrix protein obviously increased in acute inflammatory arthritis,and were related to inflammatory indexes.The MTX can reduce serum levels of cartilage oligomeric matrix protein in inflammatory arthritis and delay the destruction process of cartilage in CIA rats.

关 键 词：甲氨蝶呤 软骨寡聚基质蛋白 大鼠 

分 类 号：R593.22[医药卫生—内科学]