胶原蛋白凝胶的制备及其中药物体外扩散性考察  

Investigation of Preparation and Diffusion Rate of Drug in Collagen Protein Gel in Vitro

作　　者：刘克海[1] 董玲[1] 顾佳妮[1] 韩萍[1] 

机构地区：[1]上海海洋大学,上海市201306

出　　处：《中国药房》2011年第13期1189-1191,共3页China Pharmacy

基　　金：国家"十一五"科技支撑计划(2006BAD05A18)

摘　　要：目的:提取鱼鳞胶原蛋白,制成凝胶并考察其中药物的体外扩散性。方法:鱼鳞经胃蛋白酶提取、盐析、纯化、冷冻干燥得到胶原蛋白。以水杨酸为模型药物,加入柠檬酸溶液、甘油和乙醇混合液制备胶原蛋白凝胶,并采用琼脂扩散法以扩散系数k为指标评价其体外扩散性,同时与水杨酸水溶性和油脂性软膏进行比较。结果:所得胶原蛋白含量为91.04%;所制水杨酸胶原蛋白凝胶及水溶性和油脂性软膏扩散系数k值分别为68.11、58.04、17.67mm2·h-1。结论:所制凝胶中药物体外扩散速率较快,胶原蛋白可作为载体用于需较快释放药物的外用制剂。OBJECTIVE： To extract collagen protein from scales of fish, to prepare gel and to investigate in vitro diffusion rate of the drug in it. METHODS： The scales of fish were extracted effectively with pepsin and the collagen protein could be prepared after salting out, purification and freeze drying. Collagen protein gel was prepared using a serial mixture of citric acid solution, glycerol and ethanol and with salicylic acid as model drug. The in vitro diffusion rate of collagen protein gel was investigated by agar diffusion method using diffusion coefficient k as index, which was compared with the salicylic acid water-soluble ointment and greasy ointment. RESULTS： The content of collagen protein was 91.04%. The diffusion coefficient k of Salicylic acid collagen protein gel, salicylic acid water-soluble and greasy ointment were 68.11 mm^2.h^-1, 58.04 mm^2.h^-1, 17.67 mm^2.h^-1. CONCLUSION： Collagen gel can be used for external preparation to make drugs release faster.

关 键 词：胶原蛋白 凝胶 制备 琼脂扩散法 扩散速率 

分 类 号：R318.08[医药卫生—生物医学工程]