急进高原与平原大鼠肠道双歧杆菌的分子生物学实验对比研究  

Comparative study of molecular biology on acute high altitude and plain intestinal Bifidobacterium in rats

作　　者：单体栋[1] 邓芝云[2] 张方信[3] 邵珂[3] 段云[3] 张盼[3] 陈嘉屿[3] 汪泳[3] 

机构地区：[1]兰州大学第二临床医学院消化科,甘肃兰州730000 [2]兰州军区总医院检验科,甘肃兰州730050 [3]兰州军区总医院消化科,甘肃兰州730050

出　　处：《中国微生态学杂志》2011年第3期197-200,共4页Chinese Journal of Microecology

基　　金：全军"十一五"课题项目(06M089);军区医药卫生科研基金项目(CWS10JA17)

摘　　要：目的探讨应用荧光定量PCR法对急进高原大鼠肠道双歧杆菌数量变化的分子生物学实验研究。方法 Wistar大鼠60只,随机分为2组:平原对照组和3 848米的高原缺氧组各30只。急进海拔3 848米造成大鼠急性缺氧模型并分别于24、6、d取材,每次随机各取10只,观察肠道菌群中双歧杆菌的变化。结果高原缺氧组双歧杆菌数量明显少于平原对照组(P<0.05)。结论急进高原缺氧复杂环境下,可使肠道有益菌———双歧杆菌的数量减少,可能影响肠道微生态平衡。Objective To observe the change of intestinal Bifidobacterium in rats exposed to acute high altitude by using RT-PCR. Method 60 Wistar rats were randomly divided into plain control group and altitude 3 848 meters group,30 rats in each group.All rats except those in control group were posed acute hypoxia model at radical altitudes of 3 848 meters respectively.Each group was used on Day 2,4 and 6,in order to observe the change of the intestinal Bifidobacterium. Result After exposed to high attitude of hypoxia,the count of intestinal Bifidobacterium was significantly lower（P〈0.05）. Conclusion Exposure to radical high altitude can influence the Bifidobacterium′s number.

关 键 词：急进高原 双歧杆菌 肠道微生态 16S rDNA 荧光PCR 

分 类 号：R378.992[医药卫生—病原生物学]