正常和不同疾病儿童肠道细菌DNA含量的动态测量  

Continuous detection of intestinal bacteria DNA amount in chindren with different diseases and normal control

作　　者：黄永坤[1] 杨武[1] 曹志琅[2] 王艳丽[1] 娄俊丽[1] 姚萍[3] 

机构地区：[1]昆明医学院第一附属医院儿科,云南昆明650032 [2]昆明医学院附属昆华医院儿科,云南昆明650032 [3]昆明医学院第四附属医院儿科,云南昆明650021

出　　处：《中国微生态学杂志》2011年第3期233-236,共4页Chinese Journal of Microecology

基　　金：云南省中青年学术技术带头人后备人才培养计划资助项目(2006PY1-18)

摘　　要：目的了解正常和不同疾病儿童住院期间肠道细菌量的变化。方法收集正常儿童、足月儿、早产儿、急性淋巴细胞白血病(ALL)、过敏性紫癜(HSP)和支气管肺炎患儿住院不同时间的粪便标本,提取标本中细菌DNA,用分子生物学专用分光光度仪测量所收集标本的细菌DNA-A260值(ng/μL)。结果粪便标本中细菌的DNA-A260值足月儿组生后第1、3和7天的分别为476.53±83.52、825.38±95.84和952.73±95.57。早产儿组生后第1、3和7天的分别为377.20±93.40、560.10±93.56和710.00±94.02。ALL患儿大剂量甲氨蝶呤(HDMTX)化疗前1天、化疗后第37、天和对照组儿童的分别为2436.3±768.61、496.5±577.1、1966.6±598.3和3479.3±870.5。HSP患儿治疗前1天、治疗后第3、7天和对照组儿童的分别为2225.9±616.1、1780.3±547.42、055.6±570.2和3605.9±1096.9。支气管肺炎患儿治疗后第1、3、7天和对照组分别为2353.17±868.71、1448.47±534.81、1905.97±703.52和3433.90±897.22。结论正常儿童粪便细菌DNA-A260值比ALL、HSP和支气管肺炎患儿高,足月儿比早产儿高。治疗患儿在治疗早期细菌的DNA-A260值减低明显,治疗后期有逐渐恢复至正常的趋势。结果提示不同疾病患儿在住院和治疗期间的肠道菌群有紊乱,其紊乱程度各有不同。Objective To understand the intestinal flora in healthy children and patients with different diseases. Method The fecal samples were collected from the normal healthy children,term infants,preterm infants,as well as patients with acute lymphoblastic leukemia（ALL）,anaphylactic purpura and pneumonia.The bacteria DNA in the samples were extracted.The bacteria DNA-A260（ng/μl） was measured by biophotometer. Result The bacteria DNA-A260 in the feces of term infants were 476.53±83.52,825.38±95.84 and 952.73±95.57,respectively,at 1st,3th and 7th day after birth,while those of preterm infants were 377.20±93.40,560.10±93.56 and 710.00±94.02,respectively;The bacteria DNA-A260 of patients with ALL were 2436.3±768.6,1496.5±577.1 and 1966.6± 598.3,respectively,at 1 day before and 3 and 7 days after High-dose methotrexate treatment,compared with 3479.3±870.5 of the control;Those of pattents with anaphylactic purpara were 2225.9±616.1,1780.3±547.4 and 2055.6±570.2,respectively, at 1 day before and 3 and 7 days after treatment,compared with 3605.9±1096.9 of normal control;Those of patients with pneumenia were 2353.17±868.71,1448.47±534.81 and 1905.97±703.52,respectively,at 1st,3th and 7th day after treatment,compared with 3433.90±897.22 of the control. Conclusion The result suggest that there are the intestinal flora disorder in hospitalized children with different diseases.The disorder degree is different.

关 键 词：疾病 儿童 肠道 细菌 DNA 

分 类 号：R378[医药卫生—病原生物学]