复方六月青对肝星状细胞I型胶原合成的抑制作用  

Inhibitory effect of compound Liuyueqing on synthesis of collagen type I in hepatic stellate cells

作　　者：张士军[1] 陈兆霓[1] 张志伟[2] 李勇文[1] 林兴[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021 [2]广西中医学院附属瑞康医院,南宁530011

出　　处：《中国新药杂志》2011年第6期544-546,552,共4页Chinese Journal of New Drugs

基　　金：广西科学研究与技术开发计划(桂科攻0322024-5E);广西研究生教育创新计划(2006105981007D14)

摘　　要：目的:研究复方六月青(CLYQ)对肝星状细胞(HSC)I型胶原(Col I)合成的影响。方法:采用四甲基噻唑蓝(MTT)法检测CLYQ对细胞的毒性,分别采用免疫细胞化学法和实时荧光定量PCR法检测CLYQ对HSC的Col I蛋白和mRNA表达的影响。结果:CLYQ对HSC毒性很低。CLYQ各剂量组中HSC的Col I蛋白表达显著减弱(P<0.01)。CLYQ高、中剂量组均可显著下调HSC的Col I mRNA表达(P<0.01)。结论:CLYQ通过下调HSC的Col I蛋白及mRNA表达,从而减少细胞外基质(ECM)合成,发挥抗肝纤维化作用。Objective：To determine the effect of compound Liuyueqing（CLYQ） on the synthesis of collagen type I（Col I） in hepatic stellate cells（HSCs）.Methods：The expression of Col I protein and mRNA in HSCs was detected by immunocytochemistry and real-time fluorescence quantitative PCR,respectively.Results：Col I protein expression in the HSCs was significantly weakened after CLYQ treatment at all concentrations（P〈0.01）.The expression of Col I mRNA in HSCs was significantly down-regulated after CLYQ treatment at higher concentrations（P〈0.01）.Conclusion：CLYQ can down-regulate the expression of Col I mRNA and protein in HSCs,result in reduction of extracellular matrix（ECM） synthesis,and may play a inhibitory role in hepatic fibrosis.

关 键 词：复方六月青 肝纤维化 肝星状细胞 I型胶原 

分 类 号：R978.7[医药卫生—药品]