大鼠脊髓半横切损伤动物模型的建立与神经功能评价  

The establishment and certification of animal model of hemisected spinal cord injury in rats

作　　者：黄鹏[1] 余瑛[1] 王永堂[2] 鲁秀敏[1] 曾琳[2] 伍亚民[2] 

机构地区：[1]重庆理工大学药学与生物工程学院,400050 [2]第三军医大学大坪医院野战外科研究所,创伤、烧伤与复合伤国家重点实验室,重庆市400042

出　　处：《实用医学杂志》2011年第6期954-956,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:30600665);重庆市自然科学基金(编号:CSTC,2008BB5107);重庆市教委科学技术研究项目(编号:KJ100804);第三军医大学青年科研基金(编号:06XG048);重庆理工大学科研启动基金(编号:2008ZD21)

摘　　要：目的:建立一种稳定可靠、操作简单的脊髓半横切损伤动物模型,并对其神经功能进行初步评价。方法:将18只SD大鼠随机分为正常对照、假手术组和T10段脊髓半切组。术后不同时间点分别采用BBB运动功能评分及足迹法检测其脊髓功能的变化情况,同时检测其运动诱发电位(MEP)和体感诱发电位(SEP),记录N1及P1波潜伏期。结果:损伤组BBB运动功能评分显著低于正常对照及假手术组(P<0.01),且足迹法检测结果也存在统计学意义(P<0.01)。神经电生理检测结果表明,SEP和MEP潜伏期均具有统计学意义(P<0.01)。结论:成功制备了大鼠脊髓半切损伤动物模型,为研究脊髓损伤相关机制及药物治疗奠定了基础。Objective To set up and identify a practical and reliable animal model of spinal cord injury. Methods Sprague Dawley （SD） rats were randomly divided into three groups, normal control groups, sham operation and T10 hemisected spinal cord injury groups. The function of hind limb was observed with Basso Beaflie Bresnahan （BBB） score and footprint assay at different times and motor evoked potentials （MEP）, somatosenaory evoked potentials （SEP）, record N1 and P1 delays. Results There were statistical difference of potential period and voltage of SEP-N1 and MEP-P1 between hemisected spinal cord injury groups and normal control groups, or sham operation groups（P 〈 0.01 ）, as well as BBB score （P 〈 0.01 ） and footprint assay （P 〈 0.01 ）. Conclusion Succesaful establishment bemisection of spinal cord injury in rats, and lay a good foundation of medical treatment and the mechanism of SCI clinical application.

关 键 词：脊髓损伤 动物模型:行为检测 

分 类 号：R651.2[医药卫生—外科学]