向日葵CMS恢保关系的分子遗传学研究  

Molecular Genetic Studies on the Restoration and Maintenance in CMS Sunflower

作　　者：季静[1] 王罡[1] 吴颖[1] 王萍[1] 

机构地区：[1]解放军农牧大学植物分子生物学研究室,吉林长春130062

出　　处：《作物学报》1999年第6期683-685,共3页Acta Agronomica Sinica

基　　金：国家自然科学基金资助项目(39870449)及吉林省科委课题.

摘　　要：本研究对具有代表性的向日葵CMS品系和自交系进行了遗传学及分子生物学研究.通过分子杂交分析了来自21种CMS品系的5种具代表性的不同细胞质基因类型,发现在atpA基因位点有4种分子杂交类型;根据不同的CMS类型对20种自交系的恢保关系进行了分析;并对11种自交系的恢复基因类型进行了研究,其中6个系含有Rfl基因,5个系不含有Rfl基因.Typical CMS and inbreed lines were investigated by genetic and molecular methods. 21CMS lines were analyzed and classified into 5 types according to their cytoplasmic genes. After Southern analysis 4 kinds of patterns were discovered at atpA locus. Based on their CMS types, the relationship between restoration and maintenance in 20 inbreed lines were analyzed. Furthermore, the types of restoration genes in 11 inbreed lines were also determined. The results indicated that 6 lines contain Rf1 gene, and other 5 lines do not contain.

关 键 词：向日葵 CMS MTDNA RAPD标记 分子遗传学 

分 类 号：S565.5[农业科学—作物学]