高效液相色谱法测定复方盐酸阿米洛利血药浓度  

Determination of Amiloride and Hydrochlorothiazide in Human Plasma by HPLC

作　　者：王锋[1] 王伟佳[2] 

机构地区：[1]安徽省芜湖市妇幼保健院,安徽芜湖241000 [2]皖南医学院弋矶山医院国家药物临床试验机构,安徽芜湖241001

出　　处：《今日药学》2011年第2期81-83,共3页Pharmacy Today

摘　　要：目的建立一种反相高效液相色谱法同时测定血浆中阿米洛利和氢氯噻嗪浓度。方法样品处理采用蛋白沉淀法。色谱条件:Lichrispher C18,粒径5μm,4.6 mm×150 mm;流动相为乙腈:水:三乙胺(10∶160∶1),用磷酸调pH至2.8,流速为1.5 ml/min,柱温30℃;阿米洛利采用荧光检测,激发波长368 nm发射波长415 nm。氢氯噻嗪采用紫外检测,波长271 nm。结果阿米洛利线性范围0.5～20μg/L(r=0.999 7),最低检测浓度为0.5μg/L,批内、批间的相对标准偏差值(RSD)皆<10%。氢氯噻嗪线性范围在30～1200μg/L(r=0.999 8)最低检测浓度为30μg/L,批内、批间的RSD皆<10%。结论本法具有操作简便,分析快速准确、干扰小等特点。可用于测定阿米洛利及氢氯噻嗪的体内药代动力学研究。Objective To establish a RP-HPLC method for the determination of amiloride and hydrochlorothiazide in human plasma. Methods The plasma samples were precipitated with 10% perchloric acid, and analyzed by HPLC. Chromatographic condition of amiloride and hydrochlorothiazide was as follows： The chromatography was performed on a Lichrspher C18 column （4.6 mm×150 mm, 5 μm）, with a mobile phase consisting of acetonitrile-water-triethylamine （10： 160：1 ）, which was adjusted to pH = 2.8 with phosphoric acid, at a flow rate of 1.5 ml/min, and with column temperature 30℃; Detection of amiloride and hydrochlorothiazide were used with fluorescence and UV detector, respectively. The fluorescence detector was set at Ex 368 nm and Em 415 nm; The UV detector was set at 271 nm. Results The linear range of amiloride was 0.5 - 20 μg/L（ r = 0. 999 7 ） and limit of test was 0.5 μg/L ; within-batch RSD and inter-batch RSD were less than 10%. The Hydrochlorothiazide linear range was 30 - 1 200μg/L （r = 0. 999 8） and limit of test was 30 p.g/L; within-day RSD and inter-day RSD were less than 10%. Conclusion The method is proved to be simple, rapid, accurate, and reliable, and is suitable for the determination of amiloride and hydrochlorothiazide concentration in human plasma and pharmacokinetic studies.

关 键 词：阿米洛利 氢氯噻嗪 高效液相色谱法 

分 类 号：R969[医药卫生—药理学]