黑莓树莓杂交品种Boysen的组培快繁体系建立  

Establishment of rapid propagation technique and plantlet regeneration for blackberry×raspberry cultivar ‘Boysen’

作　　者：王小敏[1] 李海燕[1] 李维林[1] 吴文龙[1] 

机构地区：[1]江苏省中国科学院植物研究所南京中山植物园,南京210014

出　　处：《安徽农业大学学报》2011年第2期222-226,共5页Journal of Anhui Agricultural University

基　　金：江苏省农业高科技项目;江苏省农业科技自主创新资金项目(cx(10)109);江苏省科技基础设施建设计划项目(BM2009041);江苏省林业三项工程项目(lysx[2010]17)共同资助

摘　　要：为了建立黑莓树莓杂交品种‘Boysen’的组培快繁体系,以MS为基本培养基,以带芽茎段为外植体,采用单因素和正交试验方法,研究了不同取材时间对外植体培养的影响,不同种类和浓度的激素、不同光照度对‘Boysen’生长和增殖的影响,筛选‘Boysen’组织培养各阶段适宜的培养基。结果表明,‘Boysen’最佳取材时间为每年的4至5月,初代培养时只需添加0.5 mg·L-1 6-BA即可满足外植体萌发和生长的需要;最佳继代增殖培养基为MS+1.5 mg·L-1 6-BA+0.1 mg·L-1 NAA,光照度3 000 lx;最佳生根培养基为1/2 MS+0.05 mg·L-1 NAA,获得的生根组培苗的移栽成活率可达100%。The culture mediums for three stages of tissue culture and rapid propagation of ＇Boysen＇ were op-timized,and the effects of different sampling time on initial culture,different hormones and illumination intensi-ties on the shoot proliferation and growth were studied through single factor and orthogonal experiments,with MS as the basic medium,using the stem segments with auxiliary buds of ＇Boysen＇ as explants.The results show that it is optimal for sampling in April and May;in the stage of germination and growth of explants,the optimum me-dium is MS ＋ 0.5 or 1.0 mg·L-1 6-BA;the optimum medium for proliferation is MS ＋ 1.5 mg·L-16-BA ＋ 0.1 mg·L-1NAA with the highest proliferation coefficient when the illumination intensity is 3 000 lx;the optimum medium for rooting is 1/2 MS＋0.01-0.05 mg·L-1 NAA;the transplanting survival rate is 100%.

关 键 词：Boysen 茎段 组培快繁 

分 类 号：S663.2[农业科学—果树学]