中国部分地区冠耳霉的遗传多样性研究  

Genetic diversity of Conidiobolus coronatus from some regions of China

作　　者：李鲲鹏[1] 王承芳[1] 黄勃[1] 

机构地区：[1]安徽省微生物防治重点实验室安徽农业大学,合肥230036

出　　处：《安徽农业大学学报》2011年第2期238-243,共6页Journal of Anhui Agricultural University

基　　金：国家自然科学基金项目(30770008);安徽省优秀青年科技基金(08040106902);安徽省级自然科学研究重点项目(TD200708)共同资助

摘　　要：为研究来自我国不同地区冠耳霉菌株的遗传多样性,用RAPD和ISSR分子标记,对来自中国安徽、山东、贵州、山西和陕西5省的131个冠耳霉菌株（Conidiobolus coronatus）进行遗传多样性分析。筛出的4个RAPD引物和3个ISSR引物共扩增出131条带,其中多态性条带为129条,多态性比率为98.4%,遗传相似性系数范围在0.59~0.99之间,说明受试的冠耳霉具有较为丰富的遗传多样性。利用RAPD和ISSR标记的结果,采用UPGMA聚类分析方法可将绝大多数供试材料分为4大类群。根据所划分的类群,发现冠耳霉之间的亲缘关系与地理来源存在一定的相关性。研究表明,RAPD和ISSR标记可用于冠耳霉遗传多样性的研究。In order to study genetic diversity of Conidiobolus coronatus isolated from different geographical origin in China,genetic diversities of 131 C.coronatus isolates,randomly sampled from 5 Provinces（Anhui,Shandong,Gueizhou,Shanxi and Shanxi）in China,were analyzed using RAPD（random amplified polymorphic DNA） and ISSR（inter-simple sequence repeat）markers.By using 4 selected RAPD and 3 selected ISSR primers,131 DNA bands were amplified,in which 129 bands showed polymorphism with a rate of 98.4%.The coefficient ranges of genetic similarity of the RAPD and ISSR markers were 0.59-0.99,which indicated that the abundant genetic diversities existed among the tested C.coronatus.UPGMA cluster analysis showed that these isolates were divided into 4 groups,and genetic similarities among C.coronatus isolates were partially related with geo-graphical sources.Based on the results,RAPD and ISSR markers could be applied to detecting genetic diversity among C.coronatus isolates.

关 键 词：冠耳霉 遗传多样性 RAPD ISSR 

分 类 号：Q949.323.4[生物学—植物学]