四川卧龙自然保护区华西蟾蜍的核型和Ag-NORs研究  

Cytogenetic Studies on Bufo andrewsi in Wolong Nature Reserve

作　　者：董丙君[1,2] 江帆[3] 赵尔宓[1,3] 

机构地区：[1]四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610064 [2]沈阳师范大学化学与生命科学学院,沈阳110034 [3]中国科学院成都生物研究所,成都610041

出　　处：《四川动物》2011年第2期170-172,F0002,共4页Sichuan Journal of Zoology

摘　　要：采用常规骨髓细胞制片法对产于卧龙自然保护区华西蟾蜍的核型及Ag-NORs位点进行了观察和分析。结果表明卧龙自然保护区蟾蜍与大部分蟾蜍相同:2n=22,NF=44,为6+5核型模式。核型为20M+2SM,次缢痕较为明显,位于第6对染色体(其中的一条)长臂末端,NORs位于次缢痕位置即6qter。通过与我国中华蟾蜍种组的核型及Ag-NORs位点等细胞学信息比较分析,不支持卧龙自然保护区卧龙蟾蜍和沙湾蟾蜍为有效种,其可能是华西蟾蜍的次级同物异名。In this work,we present cytogenetic data on the toads in Wolong Nature Reserve for the first time.The karyotype and NORs localities were described and compared with other toads.Toads in Wolong Nature Reserve had similar cytogenetic information to the toads of Bufo gargarizans,which had 22 chromosomes：2n=22,with 6 large-sized pairs and 5 small-sized pairs,and NF=44.The karyotype was 20M＋2SM,and secondary constriction wase also observed on the end region of the long-arms of the sixth pairs of chromosomes.Ag-NORs were located at 6qter.B.wolongensis and B.kabiscbi in Wolong Nature Reserve were not valid species in comparison with other toads.

关 键 词：卧龙地区 华西蟾蜍 核型 AG-NORS 

分 类 号：Q953[生物学—动物学]