百合杂交种及亲本耐热性比较  

Comparative Study on Heat Resistance of Lily Hybrids and Their Parents

作　　者：李蕊[1] 杨利平[2] 刘雪凝[2] 

机构地区：[1]河北农业大学园林与旅游学院,河北保定071000 [2]长江师范学院生命科学与技术学院,重庆408100

出　　处：《中国农业科学》2011年第6期1201-1209,共9页Scientia Agricultura Sinica

基　　金：河北省自然科学基金项目(C2008000265)

摘　　要：【目的】分析比较百合杂交种及亲本耐热性,筛选优良的耐热性育种材料。【方法】以离体扩繁的细叶百合(承德种源)ב西伯利亚’杂交种与细叶百合(大庆种源)ב西伯利亚’杂交种及其亲本为试验材料,测定其经过不同温度(25℃、28℃、31℃、34℃、37℃、40℃)处理后抗性生理指标及电阻抗图谱参数之间的变化规律。【结果】31℃处理后,杂交种与亲本叶绿素含量及根系活力极显著上升并达到峰值,杂交种介于亲本之间或优于亲本,两个指标呈显著相关(P<0.01);37℃处理时,两杂交种游离脯氨酸显著下降至最低(P<0.05),杂交种积累量高于亲本;34℃处理时,τm显著上升并达到峰值,相对电导率显著下降,杂交种均优于父本,两指标呈显著相关(P<0.05)。【结论】细叶百合大庆种源较承德种源耐热性强,且以细叶百合大庆种源作母本比以细叶百合承德种源作母本的杂交优势明显;杂交种耐热性与母本相近,但明显优于父本。【Objective】The aim of the test is to study the heat resistance of lily hybrids and their parents,to screen excellent breeding materials.【Method】The plantlets of lily hybrids from Lilium pumilum（provenance of Chengde）×＇Siberia＇ and L.pumilum（provenance of Daqing）×＇Siberia＇ as well as their parents were used as experimental materials and they treated at different temperatures（25℃,28℃,31℃,34℃,37℃,40℃）,and then the changing regularity of resistance physiological indices and the electrical impedance spectroscopy parameters were studied.【Result】 Treatment at 31℃ promoted the chlorophyll content and root activity,hybrids were their parents or were better than parents in chlorophyll content and activity,and there was a significant correlation between the two indexes（P0.01）.At 37 ℃,the proline content of two hybrids reached the lowest（P0.05）,but still was higher than that of their parents.At 34 ℃,the relaxation time rose and the conductivity decreased significantly,the hybrids were better than male parents,the two indexes had a significant correlation（P0.05）.【Conclusion】 The heat resistance of L.pumilum（provenance of Daqing） was higher than L.pumilum（provenance of Chengde）,the heterosis of hybrid was significant when it was used as female parent.The heat resistance of hybrids was similar to their female parents,but was better than that of their male parents.

关 键 词：百合 温度处理 生理指标 电阻抗图谱参数 耐热性 种源 

分 类 号：S682.29[农业科学—观赏园艺]