芦丁对去卵巢处女大鼠乳腺发育作用的影响  

Experiment Study of Rutin on Mammary Gland Development in Ovariectomized Virgin Rats

作　　者：郭旭东[1] 刁其玉[1] 王月影[2] 屠焰[1] 闫贵龙[3] 付彤[2] 汪新建[2] 邓凯东[1] 张乃锋[1] 鞠翠芳[1] 李军涛[2] 宋凯[2] 顾小卫 吕宗友 徐俊 

机构地区：[1]中国农业科学院饲料研究所/农业部饲料生物技术重点实验室,北京100081 [2]河南农业大学/农业部动物生长发育调控重点开放实验室,郑州450002 [3]河北北方学院,河北张家口075000 [4]扬州大学,江苏扬州225009

出　　处：《中国农业科学》2011年第6期1266-1276,共11页Scientia Agricultura Sinica

基　　金：国家"十一五"科技支撑计划<农副产品饲料转化与高效利用关键技术研究>项目(2006BAD12B04-04)

摘　　要：【目的】通过考察芦丁对去卵巢处女大鼠内分泌激素水平、激素受体表达的影响以及乳腺组织形态学的观察,来研究芦丁促进乳腺发育的类雌激素样作用。【方法】试验选择32只去卵巢处女Wistar大鼠,随机分为四个处理组,假手术Sham组,每日每只灌胃生理盐水(2 mL)一次;卵巢切除Ovx组,每日每只灌胃生理盐水(2 mL)一次;芦丁Ovx+Rut组,每日每只灌服芦丁(60mg.kg-1BW)一次;雌二醇Ovx+Est组,每周每只肌注雌二醇(60μg.kg-1BW)一次。试验为期两周,试验结束后采集大鼠血液样品、乳腺组织样品等,运用放免技术检测血浆和乳腺组织中雌激素(E2)、催乳素(PRL)、生长激素(GH)的含量,RT-PCR技术检测乳腺组织这些激素受体的表达量,并进行乳腺组织形态学观察。【结果】试验结果显示,大鼠血浆中E2的水平,Ovx+Rut组和Ovx+Est组高于Ovx组(P<0.05),该两组间差异不显著(P>0.05),Ovx+Rut组水平低于Sham组(P<0.05),Ovx+Est组与Sham组差异不显著(P>0.05),乳腺组织中E2含量呈现相同规律;PRL在血浆与乳腺组织中的含量都表现为Ovx组<Ovx+Rut组<Ovx+Est组<Sham组(P<0.05),血浆中GH的含量Ovx组低于Ovx+Rut组低于Ovx+Est组低于Sham组(P<0.05),在乳腺组织GH含量,Ovx组低于Ovx+Rut组和Ovx+Est组(P<0.05),Ovx+Rut组低于Sham组(P<0.05),Ovx+Rut组和Ovx+Est组间差异不显著(P>0.05),Ovx+Est组与Sham组差异不显著(P>0.05);大鼠乳腺组织中雌激素受体ER、催乳素受体PRLR、生长激素受体GHR在Ovx组、Ovx+Rut组、Ovx+Est组、Sham组组间数值依次增大(P<0.05),表现相同变化趋势;乳腺组织形态学观察发现,Ovx+Rut组、Ovx+Est组、Sham组乳腺组织的发育显著好与Ovx组(P<0.05)。【结论】芦丁能够提高去卵巢处女大鼠E2水平,促进PRL、GH分泌,上调乳腺组织中ER、PRLR、GHR的表达量。【Objective】 The objective of this study was to investigate the regulatory effect of rutin in promoting mammary gland develop＇s estrogen-like effect by examining the effects of ovariectomized virgin rats levels of endocrine hormones,the expression of receptor and histomorphology of development.【Method】Thirty-two ovariectomized virgin rats were randomly assigned to four treatments： sham,gastric infusion of 2 ml normal saline per mouse and per day;Ovx,gastric infusion of 2 ml normal saline per mouse and per day;Ovx＋Rut,gastric infusion of 60 mg rutin？kg-1BW per day;Ovx＋Est,intramuscular injection of 60 μg estradiol？kg-1BW weekly.The trial lasted two weeks.The samples of blood,gland tissues were collected respectively.The level of estrogen（E2）,prolactin（PRL） and growth hormone（GH） in plasma and gland tissues were dected by the use of Radioimmunoassy（RIA） technology;and the expression of estrogen receptor（ER）,prolactin receptor（PRLR） and growth hormone receptor（GHR） in gland tissues were detected by the use of RT-PCR technology.Histomorphology of development about mammary gland were observed.【Result】The results showed that,the level of E2 in plasma and gland tissues was Ovx＋Rut and Ovx＋Est were highter than Ovx（P0.05）,and the difference between two groups was not significant（P 0.05）,Ovx＋Rut was lower than Sham（P0.05）,Ovx＋Est and Sham have no distinct（P0.05）.The level of E2 in gland tissues showed the same regularity.The order of the PRL in plasma and gland tissues was OvxOvx＋RutOvx＋EstSham（P0.05）.The levels of GH in plasma peresents Ovx,Ovx＋Rut,Ovx＋Est,Sham increased in turn（P0.05）.The GH In gland tissues,the levels of GH in Ovx was lower than in Ovx＋Rut and Ovx＋Est（P0.05）,and Ovx＋Rut was lower than Sham（P0.05）.The difference between Ovx＋Rut and Ovx＋Est was not distinct（P0.05）,Ovx＋Est and Sham＇s differences were not distinct either（P0.05）.The expression of ER,PRLR,GHR in gland tissues in Ovx,Ovx�

关 键 词：芦丁 大鼠 乳腺发育 激素 受体 

分 类 号：S865.12[农业科学—野生动物驯养]