再生障碍性贫血患者血浆IL-3和IL-8水平的检测  

Detection of interleukin-3 and interleukin-8 levels in plasma of aplastic anemia patients

作　　者：昝丽娜[1] 李玉云[1] 谢扬虎[1] 程佩萍[2] 江杨帆[1] 吴俊英[1] 

机构地区：[1]蚌埠医学院临床检验诊断学实验中心,安徽蚌埠233030 [2]蚌埠医学院第一附属医院检验科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2011年第3期293-295,共3页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究资助项目(2006kj392B)

摘　　要：目的:研究白细胞介素3(IL-3)和IL-8在再生障碍性贫血(aplasticanemia,AA)发病机制中的变化及临床意义。方法:采用双抗体酶联免疫吸附试验检测30例AA患者(AA组,其中PIG-A基因突变组14例,无PIG-A基因突变组16例)和20名健康志愿者(对照组)血浆中IL-3和IL-8的水平。结果:AA患者血浆中IL-3水平与对照组差异无统计学意义(P>0.05);无PIG-A基因突变组AA患者,其血浆IL-8的水平均明显高于对照组和有PIG-A基因突变组(P<0.01);PIG-A基因突变组血浆IL-8水平与对照组差异无统计学意义(P>0.05)。对AA组IL-3和IL-8水平作相关分析,显示二者无直线相关关系(P>0.05)。结论:AA患者存在细胞免疫功能紊乱,IL-8的分泌异常可能在AA的发病机制中起到一定作用,特别是IL-8的过高分泌可能与AA的发病有着密切的联系。Objective：To observe changes of interleukin-3（IL-3） and interleukin-8（IL-8） levels and discuss its significance in pathogenesis of aplastic anemia（AA）.Methods：The method of ELISA was used to detect IL-3 and IL-8 levels in plasma of 30 AA patients and 10 normal volunteers.Results：There was no significant difference of plasma IL-3 levels between the AA patients and controls（P0.05）;Compared with groups of the normal controls and the patients with PIG-A gene mutation,the levels of plasma IL-8 significantly increased in the group of AA patients without PIG-A gene mutation（P0.01）;There was no significant difference of plasma IL-8 levels between the group of AA patients with PIG-A mutation and the normal controls（P0.05）.Conclusions：There are cellular immune dysfunction in patients with AA.Abnormal levels of IL-8 might be involved in the pathogenesis of AA and high level of IL-8 may closely linked to the occurrence of AA.

关 键 词：再生障碍性贫血 白细胞介素-3 白细胞介素-8 

分 类 号：R556.5[医药卫生—血液循环系统疾病] R392.12[医药卫生—内科学]