标本因素对荧光定量PCR检测HBV DNA的影响  

Sample factors affecting fluorescence quantitative PCR detection of HBVDNA

作　　者：郝维敏[1] 刘杰[1] 杨晓春[1] 

机构地区：[1]安徽省宿州市立医院检验科,234000

出　　处：《蚌埠医学院学报》2011年第3期295-297,共3页Journal of Bengbu Medical College

摘　　要：目的:探讨标本溶血、黄疸、脂血对荧光定量PCR检测乙型肝炎病毒(HBV)DNA的影响。方法:荧光定量PCR检测无溶血、无黄疸、非脂血血清和溶血、黄疸、高脂血清标本的HBV DNA。结果:溶血、黄疸、高脂血血清与无溶血、无黄疸、非脂血血清HBV DNA定量检测结果差异均无统计学意义(P>0.05)。结论:标本溶血、黄疸、脂血对荧光定量PCR检测HBV DNA无干扰。Objective：To explore the effects of abnormal sample characters,such as hemolysis,icterus and lipemia,on fluorescence quantitative PCR（FQ-PCR） detection of HBV DNA. Methods：Levels of serum HBV DNA in hyperlipemia with hemolysis,icterus and normal serum samples were detected by FQ-PCR. Results：There were no significant differences of HBV DNA level among the groups of hyperlipemia with hemolysis,icterus and the control（P〉0.05）. Conclusions：Hemolysis,icterus and lipemia of serum sample have no interference on FQ-PCR detection of HBV DNA.

关 键 词：黄疸 溶血 脂血 荧光定量聚合酶链反应 乙型肝炎病毒 脱氧核糖核酸 

分 类 号：R442.4[医药卫生—诊断学]