高效液相色谱-荧光分析法测定人体尿液中的墨蝶呤  

Determination of sepiapterin in human urine by HPLC with fluorescence detection

作　　者：崔盼盼[1] 万益群[1,2] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学分析测试中心,江西南昌330047

出　　处：《南昌大学学报（理科版）》2011年第1期60-63,共4页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(NO.20765002);江西省自然科学基金资助项目(NO.0620049)

摘　　要：建立了人体尿液中墨蝶呤的高效液相色谱-荧光分析方法。尿液经乙腈处理,过0.45μm水相滤膜后定容,可进行液相色谱分析。色谱柱为Diamonsil C18柱,以水-甲醇(80:20,v/v)为流动相,流速为1.0 mL/min,荧光检测波长为λex=425 nm,λem=530 nm。墨蝶呤含量在0.005～1.0μg/mL范围内与色谱峰面积呈良好的线性关系,线性回归方程为y=106x-328.02(r=0.999 0),检测限是0.002μg/mL。其加标平均回收率在98.7%～106.8%之间,相对标准偏差小于7.34%。该方法简便,应用于胃癌病人和健康人尿样中墨蝶呤测定,结果较好。The determination of sepiapterin in human urine by HPLC-FLD has been studied.The urine was filtered by 0.45 μm membrane after dealed with CH3CN,then the sample was analyzed by HPLC-FLD.The chromatographic column was Diamonsil C18,the moble phase was H2O-methanol（80：20,v/v）,the flow rate was 1.0 mL/min,the excitation and emission wavelengths were 425 nm and 530 nm,respectively.The linear range of sepiapterin was 0.005-1.0 μg/mL.The linear regression equation was y=106x-328.02（r=0.999 0）,and the limit of detection was 0.002 μg/mL.The average recoveries were in the range of 98.7%～106.8% with the RSD below 7.34%.The proposed method was applied to the determination of sepiapterin in both the gastric cancer patients＇urine samples and healthy human urine with satisfactory results.

关 键 词：墨蝶呤 人体尿液 高效液相色谱 荧光检测 

分 类 号：O657.7[理学—分析化学]