鸡IL-18原核表达工程菌遗传稳定性与表达产物的生物学活性检测  

Heredity Stability and Bioactivity of Prokaryotic Engineering Bacteria Strain Expressing Chicken IL-18

作　　者：赵敏[1,2] 张春杰[1] 程相朝[1] 李银聚[1] 吴庭才[1] 杨涛[2] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]河南农业职业学院动物科学系,河南中牟451450

出　　处：《河南农业科学》2011年第2期138-141,共4页Journal of Henan Agricultural Sciences

基　　金：河南省自然科学基金项目(0511032300)

摘　　要：通过对鸡白介素18(IL-18)原核表达工程菌BL21-ChIL-18遗传稳定性、表达产物的West-ern-blot及其经Sephadex G-100纯化后的ELISA和淋巴细胞转化试验分析,以检测其遗传稳定性及其表达产物的生物学活性。结果表明,该重组工程菌能稳定地表达鸡IL-18,表达产物不仅能够与抗鸡IL-18的多克隆抗体发生反应,而且具有刺激淋巴细胞增殖并诱导其分泌γ干扰素的作用,为研究鸡IL-18在鸡体内的生物学功能奠定了基础。In order to detect the heredity stability and bioactivity of prokaryotic engineering bac-teria strain BL21-ChIL-18,its heredity stability and Western-blot analysis of its products were performed and other bioactivity of Sephadex G-100 column purified products were analyzed by ELISA and MTT.The results showed that the recombinant engineering strains can stably express chicken IL-18.Expression products can react with polyclonal antibody anti-chicken IL-18 and induce T lymphocytes proliferation and secreting IFN-γ in vitro.This laid a foundation for further research and the biological function within the chicken body studies.

关 键 词：鸡 白细胞介素18 原核表达 生物活性 

分 类 号：Q784[生物学—分子生物学]