橡胶树内生细菌多样性初探及拮抗菌株的筛选  

Rubber endophytic bacteria population diversity and screening of antagonistic strains

作　　者：时涛[1] 彭建华[1] 刘先宝[1] 戴英葵[1] 黄贵修[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737

出　　处：《中国森林病虫》2011年第2期5-9,共5页Forest Pest and Disease

基　　金：公益性行业(农业)科研专项子课题(nyhyzx07-033-2-1);海南省自然科学基金项目(807041);中国热带农业科学院博士启动经费

摘　　要：经过严格的表面消毒后,从成年橡胶树各组织中分离47份内生细菌。各组织中的内生细菌数量和种类由多到少分别为树根、花序、树皮、叶柄、叶片和果实。以橡胶树多主棒孢病菌Corynes-pora cossiicola(Berk&Curt.)Wei和炭疽病菌Colletotrichum gloeosporioides Penz.为指示菌,从中筛选出有较好作用的2个菌株,菌落形态观察、生理生化特征鉴定和16S rDNA序列分析表明它们是枯草芽孢杆菌Bacillus subtilis和莫哈韦芽孢杆菌B.mojavensis。Forty seven isolates were separated from different tissus of matured rubber after strict surface sterilization.The amounts and species of endophytic bacteria in rubber demonstrated a decreasing trend in the tissues from root,inflorescence,bark,petiole,lamina and fruit.Corynespora cassiicola and Colletotrichum gloeosporioides isolated from rubber were used as indicative pathogens and two strains with relatively strong antimicrobial effect were screened.The colony figure observation,characteristic of physiological and biochemical and sequence analysis of 16S rDNA showed the two strains were Bacillus subtilis and B.mojavensis respectively.

关 键 词：橡胶树 内生细菌 拮抗作用 

分 类 号：S763.13[农业科学—森林保护学]