大鼠禁水应激下丘脑室旁核c-fos与肾水通道蛋白2表达的变化  

The expression changes of c-fos in the paraventricular nucleus of hypothalamus and AQP2 in the kidney at stress rats during water deprivation

作　　者：侯天德[1] 周涵[1] 杨荣[2] 程昉[1] 马玉兰[1] 徐瑞[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]甘肃省肿瘤医院,甘肃兰州730050

出　　处：《西北师范大学学报（自然科学版）》2011年第2期97-101,共5页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省教育厅研究生导师科研项目(0801-06)

摘　　要：对大鼠禁水1d、2d和3d后取血,测定其血清中多种无机离子和尿素氮浓度的变化.应用免疫组织化学方法观察禁水过程中c-fos在下丘脑室旁核(PVN)中的表达及水通道蛋白2(AQP2)在肾脏中的表达.结果表明,禁水处理1d,2d和3d后各组大鼠血清中Na+,Cl-,Mg2+,P3+均呈不同程度升高,血清中Ca2+,K+离子变化不显著;尿素氮的浓度随着时间的延长而显著升高.禁水过程中各组大鼠PVN c-fos表达部位的面密度值及平均吸光度逐渐增大,随着禁水时间的延长,AQP2在肾脏集合管的表达也逐渐增强,AQP2与血清中Na+,Cl-呈正相关.The rats blood samples are collected after 1,2 and 3 days in condition of drinking water deprivation.The concentration of inorganic ions and urea nitrogen of the samples are analysed.The expression of c-fos in the paraventricular nucleus of hypothalamus and AQP2 in the kidney are measured by immunohistochemical assay.The results indicate that Na＋,Cl-,Mg2＋,P3＋ concentration of the serum are increased in various degrees and no significant changes on Ca2＋ and K＋ concentration of serum are observed at water deprived groups.Along with the time of water deprivation,the content of urea nitrogen rises significantly.The surface area density and average absorbance of c-fos immunoreactive in the paraventricular nucleus increases and AQP2 expression in the kidney collecting duct increases gradually with the time.AQP2 expression is positively correlated with Na＋,Cl-concentration of the serum.

关 键 词：禁水 下丘脑室旁核 C-FOS 水通道蛋白2 血清无机离子 

分 类 号：Q954.2[生物学—动物学]