鸡血藤提取物中缩合鞣质的含量测定及其抗肿瘤活性初步研究  

Determination on the Contents of Condensed Tannins in Spatholobus suberectus Dunn. Extracts and Primary Study on their Anti-tumor Activities

作　　者：程悦[1] 符影[1] 王志宇[2] 杨得坡[1] 陈建萍[2] 王冬梅[1] 

机构地区：[1]中山大学药学院,广东广州510006 [2]香港大学中医药学院

出　　处：《中山大学学报（自然科学版）》2011年第2期75-80,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：科技部国际合作资助项目(2009DFA31230)

摘　　要：对鸡血藤/φ=60%醇提物及不同极性溶剂萃取物中总黄酮和缩合鞣质含量进行测定,并测定它们对肿瘤细胞增殖的抑制活性。采用紫外分光光度法测定各样品中总黄酮含量;采用香草醛硫酸法测定各样品中缩合鞣质含量,结果显示,鸡血藤/φ=60%醇提物、乙酸乙酯不溶物、乙酸乙酯萃取物、正丁醇萃取物、水层留余物的总黄酮含量分别为(6.49±1.99)%、(7.36±0.13)%、(9.35±0.22)%、(8.41±0.27)%、(4.48±1.53)%;缩合鞣质含量分别为(51.09±0.37)%、(52.34±4.73)%、(73.22±2.72)%、(83.11±5.87)%、(29.74±2.12)%。采用MTT法测定各样品对乳腺癌MCF-7和结肠癌HT-29细胞增殖的抑制活性,结果不同样品对HT-29和MCF-7细胞增殖表现出不同程度的抑制活性,其中正丁醇萃取物对肿瘤细胞增殖的抑制活性最强。The contents of total flavonoids and condensed tannins in the ethanol extract of Spatholobus suberectus Dunn and its different solvents partitioned extracts were determined by ultraviolet spectrophotometry and Vanillin-H2SO4 method,respectively.The contents of total flavonoids in the φ=60% ethanol extract,ethyl acetate insoluble extract,ethyl acetate partitioned extract,n-butanol partitioned extract,water residue were（6.49±1.99）%,（7.36±0.13）%,（9.35±0.22）%,（8.41±0.27）%,（4.48±1.53）%,respectively,while their contents of condensed tannins were（51.09±0.37）%,（52.34±4.73）%,（73.22±2.72）%,（83.11±5.87）%,（29.74±2.12）%,respectively.The inhibitory activities on the proliferation of HT-29 and MCF-7 tumor cells were determined by MTT.The extract samples all showed inhibitory activities in different extent on the proliferation of HT-29 and MCF-7 tumor cells.The n-butanol partitioned extract had the strongest inhibitory activities on the proliferation of both tumor cells.

关 键 词：鸡血藤 缩合鞣质 总黄酮 抗肿瘤细胞增殖 

分 类 号：R962[医药卫生—药理学]