RGDS肽对胶元活化的大鼠血小板L-Arginine/NO途径的影响  

Effect of Arg-Gly-Asp-Ser (RGDS) Peptide on L-Arginine/NO Pathway of Collagen Activated Rat Platelet

作　　者：姚兴海[1] 王培勇[2] 于文杰 李夏[2] 苏静怡[2] 唐朝枢[2] 

机构地区：[1]首都医科大学病理生理教研室,北京100054 [2]北京医科大学心血管基础研究所,北京100083

出　　处：《基础医学与临床》1999年第5期42-45,共4页Basic and Clinical Medicine

摘　　要：本工作在胶原活化的大鼠血小板上，观察RGDS肽对血小板聚集、L一精氨酸（L－Arg）转运、一氧化氮合酶（NOS）活性和亚硝酸盐（NO2－）含量的影响。结果发现，4μg／mL胶原引起血小板聚集时，L－Arg转运增强、NOS活性增高和NO2-含量增加。血小板L－Arg转运的Vmax与NOS活性和NO2-生成呈明显正相关（r=0.8100和0.8343，p＜0．01）；血小板聚集与NO2-生成亦呈明显正相关（r=0.7660，P＜0.05）。RGDS肽200μmol／L凡与胶原共同孵育，可明显抑制胶原引起的血小板聚集、L－Arg转运增强、NOS活性增高和NO2-含量增加。提示，胶原激活的血小板L－Arg／NO途径增加是通过Ⅱb／Ⅲa复合物所介导，RGDS肽可括抗其增加作用。The aim of this work is to investigate the effect of ArgGly-Asp-Ser (RGDS) peptide on plateletaggregation, L-Arginine (L-Arg) transport, NO synthase (NOS) activity and nitrite (NO, -) contentduring platelet activation. Experiments were performed on collagen activated rat platelets in vivo.Results showed that collagen 4μg/mL, while inducing platelet aggrgation, obviously enhanced L-Argtransport, NOS activity and NO, - production. When platelets and collagen were incubated togetherwith 200 μmol/L RGDS peptide it was found that RGDS peptide markedly inhibit collagen activatedplatelet aggregation, L-Arg transport, NOS activation and NO, - content. The Vmax of L-Argtransport is positively related with NOS activation and NO, - production (r=0.8100 and 0.8343,P<0.0l), The platelet aggregaion and NO, - production are also in positive relationship (r=0.7660,P<0.05) , The resultS suggested that the L-Arg/NO pathway ofcollagen activated platelet is enhanced byGP Ⅱ b/ Ⅲ complex medium and the increase of the L-Arg/NO pathway can be resisted by RGDS.

关 键 词：RGDS肽 血小板聚集 精氨酸转运 胶元 NO 

分 类 号：Q516[生物学—生物化学] Q977.6