基于线粒体Cytb基因的10种石珊瑚的系统发育关系  

Phylogenetic Relationships in 10 Species of Scleractinian Coral Based on Cyt b Sequences

作　　者：刘丽[1] 陈育盛[1] 李晓娜[1] 申玉春[1] 刘楚吾[1] 

机构地区：[1]广东海洋大学水产学院南海水产经济动物增养殖广东省普通高校重点实验室,广东湛江524025

出　　处：《广东海洋大学学报》2011年第1期6-11,共6页Journal of Guangdong Ocean University

基　　金：国家海洋公益性行业科研专项(201105012);广东省海洋渔业科技推广专项(A200908F01);广东省自然科学基金(8452408801001272)

摘　　要：通过PCR扩增直接测序得到10种徐闻石珊瑚线粒体Cyt b基因部分序列,分析其碱基组成和变异频率,并采用Neighbor-Joining和Maximum Parsimony法构建系统发育树。结果显示:序列中A+T比例为61.0%,G+C比例为39.0%,前者明显高于后者,碱基替换主要发生在密码子第3位;滨珊瑚科分类与传统分类存在一定差异,揭示传统形态学分类可能受珊瑚骨骼生长可塑性限制,造成分类不准确;刺柄珊瑚属与蜂巢珊瑚科各属亲缘关系较近。Partial sequences of Cyt b gene from 10 species of Scleractinian coral were amplified by polymerase chain reaction（PCR） and directly sequenced.Base ratio and variations were analyzed,and the NJ tree and MP tree were constructed.The result suggests the percentage of A＋T is 61%,obviously higher than that of G＋C,39%,and replacement of nucleotide mostly occurs at the third cod.In the Poritidae,classification is not in accordance with that of traditional taxonomy,and display morphological classification may be limited by coral skeletons plasticity.Hydnophora is most closely related to Faviidae.

关 键 词：石珊瑚 CYTB基因 碱基比例 变异频率 系统发育树 

分 类 号：Q953[生物学—动物学]