注射黄芪多糖对吉富罗非鱼c型溶菌酶基因表达量的影响  

Effect of Astragalus Polysaccharides (APS) on the Expression of Lysozyme-c Gene in GIFT Strain of Nile Tilapia(Oreochromis niloticus)

作　　者：汤菊芬[1] 吴灶和[1] 简纪常[1] 鲁义善[1] 王蓓[1] 李楠[1] 

机构地区：[1]广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524025

出　　处：《广东海洋大学学报》2011年第1期58-61,共4页Journal of Guangdong Ocean University

基　　金：广东省农业科技攻关项目(2008A020100014)

摘　　要：将黄芪多糖(APS)用无菌生理盐水配制成2 mg/mL和20 mg/mL针剂,腹腔注射吉富罗非鱼,以注射无菌生理盐水为对照。24 h后分别提取吉富罗非鱼鳃、头肾、肝脏、脾脏等组织中的总RNA并反转录成cDNA,利用Real-time PCR方法对不同组织中基因表达进行定量分析。结果表明:吉富罗非鱼腹腔注射20 mg/mL高剂量APS后,其鳃、头肾、肝脏等三个组织中的Lysozyme-c基因表达量显著高于对照组(P<0.05);注射2 mg/mL低剂量APS后,Lysozyme-c基因表达量仅在脾脏中出现显著上调(P<0.05)。APS可通过诱导Lysozyme-c基因在鳃、头肾、肝脏和脾脏等组织在的表达量,来提高吉富罗非鱼的机体免疫力。GIFT Strain of Nile tilapia（Oreochromis niloticus） were injected intraperitoneally with APS dissolved by sterile saline at the doses of 2mg/mL,20 mg/mL.The effects of APS injection on gene expression of lysozyme-c gene in gill,head kidney,liver and spleen of GIFT strain of Nile tilapia were determined by using quantitative real-time polymerase chain reaction.After injection of APS,high dose of APS could be found up regulated lysozyme-c gene expression in the gill,kidney and liver,and low dose of APS could be found up regulated lysozyme-c gene expression in spleen.These results indicated that APS could induced the expression of lysozyme-c gene in different organs and enhance the immune function of GIFT srain of Nile tilapia.

关 键 词：黄芪多糖 吉富罗非鱼 c型溶菌酶基因 

分 类 号：S948[农业科学—水产养殖]