HPLC法测定复方红景天胶囊中红景天苷的含量  

Determination of salidroside in compound hongjingtian capsules by HPLC

作　　者：方丽波[1] 李成网[2] 张洁[2] 

机构地区：[1]安徽中医学院药学院,安徽合肥230031 [2]安徽省医学科学研究院,安徽合肥230061

出　　处：《安徽医药》2011年第3期310-311,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立复方红景天胶囊中红景天苷的含量测定方法。方法样品采用甲醇超声提取的方法制备。色谱条件采用Shim-Pack ODS柱(150 mm×4.6 mm5,μm),甲醇-水(10∶90)为流动相,检测波长为223 nm。结果红景天苷浓度在9.61～96.08 mg.L-1范围内与峰面积值呈良好的线性关系(r=0.999 9)。回收率为97.34%,RSD=1.02%(n=6)。结论该法准确、可靠、稳定,可作为复方红景天胶囊的定量分析方法。Aim To develop a method for determination of salidroside in compound hongjingtian capsules.Methods The sample was prepared in methonal by ultrasonic extraction.The analysis was performed on a Shim-Pack ODS column,with a mobile phase of methanol-water（10∶90）,the detection wavelength set at 223 nm.Results The linearity of the concentration of salidroside and its peak area was fine in the range of 9.61 ～96.08 mg·L-1（r=0.999 9）.Recovery of salidroside was 97.34%,and RSD was 1.02%.Conclusion The method is accurate,reliable,stable and it can be used for the quantitative analysis of salidroside in compound hongjingtian capsules.

关 键 词：复方红景天胶囊 红景天苷 HPLC 

分 类 号：R286.0[医药卫生—中药学]