慢性高原病患者骨髓组织HIF-1α及VEGF表达水平和MVD变化  

Study on Expressions of HIF-1α,VEGF and Level of MVD in Bone Marrow of Patients with Chronic Mountain Sickness

作　　者：张易青[1] 袁霞[2] 李占全[2] 崔森[2] 柴克霞[2] 冀林华[2] 

机构地区：[1]青海大学附属医院病理科,810001 [2]青海大学附属医院血液科,810001

出　　处：《高原医学杂志》2010年第4期9-14,共6页Journal of High Altitude Medicine

摘　　要：目的:了解HIF-1α及VEGF在慢性高原病(CMS)骨髓组织中的表达和MVD的变化,探讨血管新生在CMS发生、发展中的作用。方法:应用免疫组织化学SP法检测20例CMS患者(CMS组)及15例长期居住西宁地区健康人(对照组)骨髓组织中HIF-1α及VEGF的表达情况和MVD的变化(用CD34单抗标记的血管计数法)并进行比较分析。结果:CMS组HIF-1α及VEGF阳性表达率分别为80%及75%,均显著高于对照组(分别为26.67%及33.33%)(P<0.05);CMS组MVD为31.93±6.13,也明显高于对照组(24.94±6.18)(P<0.01)。CMS组HIF-1α阳性表达率、VEGF阳性表达率、MVD及Hb相互间均呈显著正相关(均为P<0.05)。结论:CMS骨髓组织中HIF-1α和VEGF表达升高,MVD增加,提示HIF-1α与VEGF协同促进血管生成和红细胞增殖,参与CMS的发生发展。Objective：To research expressions of HIF-1α andVEGF,and change of micro-vessel density（MVD） in bone marrow of patients with CMS to determine effects of angiogenesis in occurrence and development of chronic mountain sickness（CMS）.Methods：The samples of bone marrow were obtained from 20 patients with CMS（CMS group） and 15 healthy adults（control group） in this study.The expressions of HIF-1α and VEGF were detected by SP immuno-histochemistry technique,and the MVD was measured using blood count（vascular endothelial cells labeled by anti-CD34 monoclonal antibody） method.The results were analyzed comparatively.Results：The positive rates of HIF-1α and VEGF in CMS group were 80% and 75%,respectively;and 26.67% and 33.33% in control group.There were significant differences in the positive rates of HIF-1α and VEGF between the two groups,（P0.05）.Level of MVD（31.93±6.13） in CMS group was significantly higher than that in control group（24.94±6.18）（P0.01）.In the CMS group,the positive rates of HIF-1α,VEGF,MVD and Hb were significantly positive iner-correlated（P0.05）.Conclusions：The over expressions of HIF-1α,VEGF and increased MVD in bone marrow of patients with CMS suggests that the HIF-1α and VEGF could promote angiogenesis and synergistic proliferation of red blood cells,and would involve in the occurrence and development of CMS.

关 键 词：慢性高原病 缺氧诱导因子-1Α 血管内皮生长因子 微血管密度 

分 类 号：R594.3[医药卫生—内科学]