清毒饮对K562细胞bcr/abl mRNA基因表达的影响  

Influence of Qingdu decoction on expression of bcr/abl mRNA in K562 Cells

作　　者：梁毅[1] 葛志红[2] 丘和明[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广东省中医院,广东广州510120

出　　处：《湖南中医药大学学报》2010年第11期31-33,共3页Journal of Hunan University of Chinese Medicine

基　　金：广东省科技计划项目(2007B031401010)

摘　　要：目的观察不同药物浓度清毒饮对人白血病细胞株K562细胞bcr/abl基因表达水平的影响,为临床应用清毒饮提供依据。方法制备不同药物浓度清毒饮(5、10、20 mg/mL)含药血清并处理K562细胞48 h,提取细胞总RNA,采用实时荧光定量RT-PCR技术分析各组培养细胞bcr/abl mRNA表达的水平。结果清毒饮中、高2种浓度清毒饮含药血清与对照组比较,对人白血病K562细胞株bcr/abl融和基因表达水平具有显著影响,差异有统计学意义(P<0.05,0.01),且呈浓度依赖性。结论清毒饮具有抑制人白血病K562细胞株bcr/abl mRNA表达的作用,提示清毒饮具有从分子水平抗肿瘤细胞增殖的效应。Objective To study the effects of Qingdu decoction in various concentrations on the expression of bcr/abl mRNA in K562 cells and provide the basis of experiment for clinic.Methods The contain-drugs serum in different concentrations of 5,10 and 20 mg/mL were made and K562 cells were treated in 48 hours and expressions of bcr/abl were analyzed with real-time quantitative reverse transcription PCR（RQ-PCR） method.Results Compared with control group,the expressions of bcr/abl of K562 cells in middle and high doses groups were significantly influenced（P〈0.05 or P〈0.01） and the declining was in a concentration-dependent.Conclusion Qingdu decoction can arrest the expression of bcr/abl of K562 cells,which indicates that Chinese drugs formula is effective for anti-proliferation of tumor cell in molecular biology level.

关 键 词：清毒饮 慢性粒细胞性白血病 K562细胞 BCR/ABL mRNA 大鼠 七叶一枝花 白花蛇舌草 

分 类 号：R285.5[医药卫生—中药学]