Ring chromosome aberrations in human lymphocytes induced by 5-20 Gy carbon ion irradiation  

作　　者：Wang Zhuanzi Li Wenjian Wang Biqian Xing Junzhen 

机构地区：[1]Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China [2]Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China [3]Xiangning Hosp Shanxi Prov, Xiangning 042100, Peoples R China

出　　处：《Nuclear Science and Techniques》2010年第6期362-365,共4页核技术（英文）

基　　金：Supported by National Natural Science Foundation of China(10875153);The President Scholarship of Chinese Academy of Sciences for Postgraduate students (O906350YZ0);The Talent Training Program for PhD in West China of the Chinese Academy of Sciences(O806260XB0,and O806270XBB)

摘　　要：In this paper,we study the biological response induced by heavy ions irradiation of high dose,human peripheral blood lymphocytes are irradiated in vitro by the carbon ions of LET=35 keV/μm,and the chromosome aberrations at absorbed doses of 0-20 Gy are analyzed by the calyculin A-induced premature chromosome condensation(PCC).The frequencies of PCC-rings at the stage of G2/M-phase increase steeply with radiation doses up to 20 Gy at a rate of 0.017 Gy^(-1).The G2-PCC index remains more than 5%up to 15 Gy,and 3%after 20 Gy,this is high enough to score a substantial number of chromosome spreads without dose-rate effect.The results show that the calyculin A-induced PCC technique is suitable for analyzing the chromosome damage induced by carbon ions irradiation of high dose.In this paper,we study the biological response induced by heavy ions irradiation of high dose,human peripheral blood lymphocytes are irradiated in vitro by the carbon ions of LET=35 keV/μm,and the chromosome aberrations at absorbed doses of 0-20 Gy are analyzed by the calyculin A-induced premature chromosome condensation（PCC）.The frequencies of PCC-rings at the stage of G2/M-phase increase steeply with radiation doses up to 20 Gy at a rate of 0.017 Gy^（-1）.The G2-PCC index remains more than 5%up to 15 Gy,and 3%after 20 Gy,this is high enough to score a substantial number of chromosome spreads without dose-rate effect.The results show that the calyculin A-induced PCC technique is suitable for analyzing the chromosome damage induced by carbon ions irradiation of high dose.

关 键 词：淋巴细胞染色体 碳离子辐照 染色体畸变 外照射 人外周血淋巴细胞 PCC技术 染色体损伤 生物反应 

分 类 号：X591[环境科学与工程—环境工程] O571.6[理学—粒子物理与原子核物理]