微囊藻毒素-LR致小鼠蛋白质氧化损伤的作用  

Protein oxidative damage in mice induced by microcystin-LR

作　　者：段丽菊[1] 张慧珍[1] 李博[1] 冯亚静[1] 程学敏[1] 杉浦则夫[2] 张振亚[2] 崔留欣[1] 

机构地区：[1]郑州大学公共卫生学院环境卫生学教研室,郑州450001 [2]日本筑波大学生命环境科学研究院水环境生态工学研究室

出　　处：《郑州大学学报（医学版）》2010年第6期953-955,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：郑州市科技局中日国际合作基金资助项目083SGHH33292-4

摘　　要：目的:探讨微囊藻毒素-LR对小鼠肝、肾和睾丸组织蛋白质的氧化损伤程度。方法:用3.0、6.0和12.0μg/kg3种不同浓度的微囊藻毒素-LR对小鼠进行腹腔注射染毒(n=5),染毒时间为7d,每天1次,并设阴性对照组。用2,4-二硝基苯肼比色法测定3种组织的蛋白质羰基含量。结果:随着微囊藻毒素-LR剂量的升高,小鼠肝、肾和睾丸的蛋白质羰基含量升高(F分别为44.631、21.975和23.962,P均<0.001)。3.0μg/kg染毒组肝、肾蛋白质羰基含量[(3.72±0.23)和(3.99±0.48)mmol/kg]高于阴性对照组[(2.90±0.22)和(3.27±0.23)mmol/kg](P均<0.05)。3.0μg/kg染毒组睾丸蛋白质羰基含量[(1.91±0.25)mmol/kg]与阴性对照组[(1.69±0.27)mmol/kg]相比,差异无统计学意义(P>0.05)。6.0μg/kg染毒组肝、肾和睾丸蛋白质羰基含量[(4.55±0.45)、(4.44±0.53)和(2.53±0.24)mmol/kg]和12.0μg/kg染毒组肝、肾和睾丸蛋白质羰基含量[(5.62±0.55)、(5.67±0.60)和(3.15±0.41)mmol/kg]均高于阴性对照组(P均<0.01)。结论:微囊藻毒素-LR对小鼠肝、肾和睾丸组织的蛋白质有氧化损伤作用。Aim：To expore the degree of protein oxidative damage on liver, kindey and testicle of mice induced by microcystin-LR.Methods：A total of 20 mice were intraperitoneally administered microcystin-LR at doses of 0,3.0, 6.0 or 12.0 μg/kg body weight every day for 7 days.The protein carbonyl content in liver,kidney and testicle tissue was measured using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage.Results：With the increase of microcystin-LR concentration protein carbonyl content in three kinds of tissue also increased（F was 44.631, 21.975 and 23.962,P0.001）. Protein carbonyl contents of liver and kidney in 3.0 μg/kg microcystin-LR exposure group [（3.72±0.23） and （3.99±0.48） mmol/kg] were higher than those of control group [（2.90±0.22） and （3.27±0.23） mmol/kg] （P0.05）. There was no significant difference in protein carbonyl content of testicle between 3.0 μg/kg microcystin-LR exposure group [（1.91±0.25） mmol/kg] and control group [（1.69±0.27） mmol/kg] （P0.05）. Protein carbonyl content of liver, kidney,and testicle in 6.0 μg/kg microcystin-LR exposure group [（4.55±0.45）, （4.44±0.53）, （2.53±0.24） mmol/kg] and 12.0 μg/kg microcystin-LR exposure group [（5.62±0.55）, （5.67±0.60）,（3.15±0.41） mmol/kg] were higher than those of control group（P0.01）.Conclusion： Microcystin-LR could induce protein oxidative damage of liver, kidney and testicle in mice.

关 键 词：微囊藻毒素-LR 蛋白质氧化损伤 蛋白质羰基含量 小鼠 

分 类 号：R994.6[医药卫生—毒理学]