藁本内酯调控MAPK信号通路的血管平滑肌细胞增生  

Regulation of vascular smooth muscle cells proliferation in MAPK signaling pathway by ligustilide

作　　者：卢群[1] 罗少洪[1] 

机构地区：[1]广东药学院基础学院,广东广州510006

出　　处：《广东药学院学报》2010年第6期632-634,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金项目(8451022401000547);广东省科技计划项目(2010B031600292);广东省科技计划项目(粤科规划字(2009)198号);广东省中医药局项目(2007365)

摘　　要：目的从细胞信号通路方面研究藁本内酯抑制体外培养的鼠血管平滑肌细胞增生的机制。方法采用MTT法测定细胞生长状况;荧光探针法、Western印迹法检测细胞内活性氧(ROS)变化、ERK和P38基因的表达活性。结果藁本内酯在40μg/mL质量浓度下作用2 d抑制率达50%;胞内ROS下降;ERK、P38表达活性下降。结论藁本内酯能有效抑制鼠平滑肌细胞增生,通过抑制ROS生成、下调P38、ERK表达从而使细胞滞留在G0/G1期。Objective To investigate the proliferation of vascular smooth muscle cells（VSMCs）by regulating MAPK signaling pathway.Methods MTT assay,fluorescent staining and flow cytometry technique were used to measure the cells inhibited by ligustilide,the reactive oxygen species（ROS）,and the activation of ERK and P38 expression.Results Statistic analysis showed that the inhibitive rate was about 50% at by ligustilide the dose of 40 μg/mL,ROS production was decreasing,and the activation of ERK and P38 expression was inhibited.Conclusion Ligustilide significantly inhibited VSMCs,which was associated with the suppression cells from G1 to S phase progress by decreasing ROS production and inhibited activation of ERK and P38 expression.

关 键 词：藁本内酯 平滑肌细胞 增生 MAPK信号 

分 类 号：R285[医药卫生—中药学]