不同温度、pH值和接种浓度对青枯菌在尾巨桉苗木根部吸附和侵入的影响  

Influences of Temperatures,pH Values and Inoculum Concentrations on the Adsorption and Ingression of Ralstonia solanacearum to Eucalyptus urophylla × E.grandis Seedling Root

作　　者：王胜坤[1] 王军[2] 康丽华[1] 徐大平[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广州510520 [2]华南农业大学林学院,广州510640

出　　处：《林业科学》2010年第12期80-85,共6页Scientia Silvae Sinicae

基　　金：国家"十一五"科技支撑计划课题(2006BAD32B02)"速生纸浆林可持续经营管理技术研究与示范";国家自然科学基金(30471395)"青枯菌对桉树的识别侵染及其生化机制研究"

摘　　要：通过测定青枯菌在尾巨桉根表吸附量和根部侵入量,研究不同温度、pH值和接种浓度对青枯菌在尾巨桉苗木根部吸附和侵入过程的影响。结果表明:青枯菌在尾巨桉苗木根部吸附侵入的最适温度条件是30℃;pH值为6.0或7.0时均有利于青枯菌在尾巨桉根表的吸附侵入;在接种浓度为3.0×108和3.0×109个.mL-1时青枯菌的吸附量和侵入量都比较高,高浓度的细菌有利于青枯菌在尾巨桉根部的吸附侵入,但过高浓度的细菌,其吸附量并不继续增加。说明在30℃,pH6.0或7.0和接种浓度高于3.0×108个.mL-1的条件,有利于青枯菌在尾巨桉根部吸附和侵入。This experiment,the influences of temperatures,pH values and inoculum concentrations on the number of adsorption and ingression of Ralstonia solanacearum to Eucalyptus urophylla × E.grandis root.The optimum temperature for R.solanacearum to adsorb to and ingress in the Eucalyptus root was 30 ℃.The more suitable pH value for the adsorption and ingression was 6.0 or 7.0.Number of the adsorption and ingression was very high under both 3.0 × 108units.mL-1 and 3.0 × 109 units.mL-1 inoculum concentrations.Higher concentration of R.solanacearum was favorable for its adsorption and ingression,but the number of adsorption and ingression did not increase accordingly when the concentration of bacteria was too high.The best conditions for R.solanacearum adsorption and ingression were 30 ℃,pH value 6.0 or 7.0 and 3.0 × 108 units.mL-1 bacterial concentration or higher.

关 键 词：青枯菌 尾巨桉 温度 PH值 接种浓度 吸附 侵入 

分 类 号：S792.39[农业科学—林木遗传育种]