不同株系拟松材线虫对黄山松和黑松苗木的致病性  

Pathogenicity of Different Isolates of Bursaphelenchus mucronatus to Pinus taiwanensis and P. thunbergii Seedlings

作　　者：陈凤毛[1,2] 史延梅[1] 王姝颖[1] 汤坚[3] 孙翠云[1] 叶建仁[1,2] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]江苏省有害生物入侵预防与控制重点实验室,南京210037 [3]安徽省林业厅,合肥230001

出　　处：《林业科学》2010年第12期86-90,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30771728);南京林业大学引进高层次人才科研基金项目(G2009-09)

摘　　要：采用23个不同来源的拟松材线虫株系对2年生黄山松和2年生黑松进行人工接种试验。结果表明:1)对黄山松苗具有致病作用的拟松材线虫有4个株系,分别为CFS4,SLD3,ZJ1和KOR1。其中接种ZJ1,SLD3 2个株系的黄山松苗的死亡率均为16.7%。2)对黑松具有致病作用的拟松材线虫有9个株系,分别是CFL1,SFS1,SFS4,SLD9,SDZ1,ANL5,ANL7,AHS9和HYC1。感病指数最高的ANL5达到83.3。其次是AL7,感病指数为54.2。发病率最高的拟松材线虫株系同样是ANL5,发病率达100%。死亡率最高的拟松材线虫系是ANL5和ANL7,达50%。3)根据拟松材线虫对2年生黑松苗的感病指数、发病率、死亡率等指标,将供试的23个拟松材线虫株系划分为3个致病等级:Ⅰ级(强致病性):ANL5和ANL7个株系;Ⅱ级(弱致病性):CKL1,SFS1,SFS4,SLD9,SDZ1,AHS9和HYC1 7个株系;Ⅲ级(无致病性):ANL1,JLH1,JLH10,JXY7,JYX31,CFL4,SLD3,YWF1,GBL3,ZJ1,HN1,TW1,JAP1和KOR1 14个株系。4)来自安徽的4个拟松材线虫株系,有3个株系对2年生黑松苗具有致病作用,其中2个株系(ANL5,ANL7)具有强致病作用;来自四川的5个拟松材线虫株系,有4个株系(SFS1,SFS4,SLD9,SDZ1)对2年生黑松苗具有中等强度的致病作用,而另外1个株系(SLD3)对2年生的黄山松苗具有致病作用;来自江苏的4个拟松材线虫株系(JLH1,JLH10,JXY7,JYX3)对2年生黄山松苗、2年生黑松苗均无致病性。Twenty-three strains of Bursaphelenchus mucronatus were used for inoculating two-year-old seedlings the following of Pinus taiwanensis and Pinus thunbergii to investigate their pathogenicity in this study.The main results were： 1）Four of Twenty-three B.mucronatus isolates had pathogenicity on the two-year-ole seedlings of P.taiwanensis,and they were SFS4,ZJ1,KOR1 and SLD3 respectively.2） Nine of Twenty-three isolates of B.mucronatus had pathogenicity on the two-year-old seedlings of P.thunbergii,and they were CFL1,SFS1,SFS4,SLD9,SDZ1,ANL5,ANL7,AHS9 and HYC1 respectively.3） According to disease index,morbidity rate and disease rate of B.mucronatus to two-year-old seedlings of P.thunbergii,twenty-three isolates of B.mucronatus were divided into three levels： Class Ⅰ（strong pathogenicity）,Class Ⅱ（weak pathogenicity） and Class Ⅲ（no pathogenicity）.4） Three of four populations from Anhui province had pathogenicity to two-year-old seedlings of P.thunbergii,wmong which two populations（ANL5 and ANL7） had strong pathogenicity;Four（SFS1,SFS4,SLD9,SDZ1） of five populations from Sichuan province had pathogenicity to two-year-old seedlings of P.thunbergii,and another population（SLD3） had pathogenicity to two-year-old seedlings of P.taiwanensis;All four populations from Jiangsu province had no pathogenicity to two-year-old seedlings of P.thunbergii and P.taiwanensis

关 键 词：拟松材线虫 致病性 黑松 黄山松 

分 类 号：S763.18[农业科学—森林保护学]