甲基磺酸甲酯处理的豫杂一号泡桐丛枝病幼苗的生长及SSR分析  

Growth of Paulownia Witches’Broom Seedlings Treated with Methylmethane Sulphonate and SSR Analysis

作　　者：翟晓巧[1] 曹喜兵[1] 范国强[1] 

机构地区：[1]河南农业大学泡桐研究所,郑州450002

出　　处：《林业科学》2010年第12期176-181,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金(30271082;30571496);高等学校博士学科点专项科研基金(20050466003);中国博士后基金(20070410874)

摘　　要：DNA甲基化在基因表达、细胞分化以及系统发育中起着重要的调节作用（陆光远等,2005;Meyeret al.,1994;Ulian et al.,1996;Rossi et al.,1997）。在植物生长发育过程中,DNA甲基化水平过高或过低,都会导致植物形态发生异常。Effects of methyl methane sulphonate（MMS） on the morphological changes of the seedlings of Paulownia tomentosa × P.fortunei with witches’broom and the phytoplasma existence were investigated.The treated seedlings were analyzed with SSR method.The results indicated that MMS treatment was able to morphologically eliminate the diseased symptom of the diseased seedlings,but growth of the seedlings decreased if MMS concentration was over 60 mg.L-1.Moreover,there still existed the phytoplasma in the infected seedlings after being treated with 20 mg.L-1 MMS,and the digestion sites of those DNA from the healthy,diseased and the diseased seedlings treated with 20 and 100 mg.L-1 MMS with SSR analysis were all the same.

关 键 词：豫杂一号泡桐 丛枝病 甲基磺酸甲酯 形态变化 SSR 

分 类 号：S763.14[农业科学—森林保护学]