孕早期氯胺酮麻醉对子代大鼠海马c-fos mRNA和c-jun mRNA表达的影响  

Effect of ketamine anesthesia in early pregnancy on the c-fos mRNA and c-jun mRNA expression in offsprings of rats

作　　者：李钢[1] 赵为禄[2] 罗佛全[2] 

机构地区：[1]江西省妇幼保健院麻醉科,南昌市330006 [2]南昌大学第一附属医院麻醉科

出　　处：《中华麻醉学杂志》2010年第11期1333-1335,共3页Chinese Journal of Anesthesiology

基　　金：基金项目：国家自然科学基金（81060093）;江西省科技支撑计划重点项目（2009BSA09700）

摘　　要：目的 探讨孕早期氯胺酮麻醉对子代大鼠海马c-fos mRNA和c-jun mRNA表达的影响.方法 孕5～13 d的SD大鼠30只,体重250～300 g,随机分为2组（n=15）：对照组（C组）和氯胺酮组（K组）.K组经尾静脉注射氯胺酮20 mg/kg,随后以130 mg·kg-1·h-1的速率静脉输注2 h;C组以等量生理盐水替代氯胺酮.子代大鼠于出生后20和30 d时测定认知功能,取海马组织,测定c-fosmRNA和c-jun mRNA表达水平并观察超微结构.结果 与C组比较,K组子代大鼠出生后30 d时认知功能测定第2天逃避潜伏期延长（P＜0.05）,海马c-fos mRNA和c-jun mRNA的表达水平差异无统计学意义,出生后20 d上述指标差异无统计学意义（P＞0.05）.K组海马神经元发生损伤.结论 孕早期氯胺酮麻醉抑制子代大鼠认知功能的机制与海马神经元受损有关,但与海马c-fos mRNA和c-jun mRNA表达无关.Objective To investigate the effect of ketamine anesthesia in the early pregnancy on the c-fos mRNA and c-jun mRNA expression in the offsprings of rats. Methods Thirty pregnant SD rats at 5-13 days of gestation were randomly divided into control group and ketamine group （n = 15 each）. Ketamine 20 mg/kg was injected intravenously through tail vein followed by 2 h infusion at a rate of 130 mg·kg-1 ·h-1 in ketmine group.While the equal volume of normal saline was given instead of ketamine in control group. The learning and memory function of the offsprings were tested by Morris water maze test on postnatal day 20 and 30. The hippocampal tissues were taken to detect the expression of c-fos mRNA and c-jun mRNA and to observe the ultrastructure. Results Compared with group C, the escape latency was significantly prolonged at 2 days during the test which was performed on postnatal day 30, but there was no significant difference in the expression of c-fos mRNA and c-jun mRNA on postnatal day 20 and 30 and in the indices mentioned above on postnatal day 20 in ketamine group （P 〉0.05）. The damage to hippocampal neurons happened in ketamine group. Conclusion The mechanism by which ketamine anesthesia in the early pregnancy inhibits the cognitive function of the offsprings is related to the hippocampal neuron damage, but not related to the expression of c-fos mRNA and c-jun mRNA in hippocampus.

关 键 词：氯胺酮 妊娠初期 基因 fos 基因 jun 

分 类 号：R285.5[医药卫生—中药学]