软骨藻酸多克隆抗体的制备  

Preparation of polyclonal antibodies against domoic acid

作　　者：刘元嫄[1] 程金平[1] 高利利[1] 徐玮[1] 吴浩东[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240

出　　处：《海洋科学》2010年第12期67-69,74,共4页Marine Sciences

基　　金：上海科委科技攻关项目(08DZ1206302)

摘　　要：为建立软骨藻酸(DA)的免疫检测法,本研究采用活泼酯法将DA与载体蛋白KLH(BSA)偶联形成完全免疫抗原(包被抗原),并通过紫外光谱扫描证明偶联成功与否。用完全免疫抗原DA-KLH对新西兰白兔进行免疫,间接ELISA法测定血清效价。实验结果表明偶联成功,经过19周免疫后血清效价达到1:1600,纯化后获得效价为1:800的多克隆抗体,同时也间接证明了合成的完全抗原具有免疫原性,为以后建立免疫分析法奠定了基础。To develop an immunoassay of domoic acid（DA）,DA was coupled to keyhole limpet hemocyanin（KLH） or bovine serum albumin（BSA） by the activation ester method.The conjugates were characterized by UV absorp-tion.Polyclonal antibodies for domoic acid were generated from rabbits after the animals had been immunized by DA-KLH for 19 weeks.The titer of antiserum was 1：1600,detected by an indirect ELISA.After purification,the titer of antiserum became 1：800.DA-KLH was proved to be a suitable immunogen to prepare antibodies against DA.

关 键 词：软骨藻酸 多克隆抗体 活泼酯法 

分 类 号：R392.33[医药卫生—免疫学]