来氟米特对狼疮肾炎患者外周血单个核细胞分泌细胞因子谱的影响  

Effects of leflunomide on the spectrum of inflammatory cytokines secreted by the peripheral blood mononuclear cells in patients with lupus nephritis

作　　者：董光富[1] 李玲[1] 张晓[1] 张光锋[1] 

机构地区：[1]广东省医学科学院．广东省人民医院风湿免疫科, 广州市510180

出　　处：《实用医学杂志》2010年第24期4491-4493,共3页The Journal of Practical Medicine

基　　金：广东省自然科学基金资助项目(编号:04300905)

摘　　要：目的:了解来氟米特对狼疮肾炎(lupus nephritis,LN)患者外周血单个核细胞分泌细胞因子水平(IL-12,IFN-r,IL-4和IL-6)有无影响。方法:取受试者空腹静脉血15mL,肝素钠抗凝,提取PBMCs,分组培养,健康对照:空白培养,植物血凝素(PHA)刺激培养;LN组:空白培养,PHA刺激培养,PHA+来氟米特体内活性产物(A771726)培养。ELISA检测健康对照和LN患者PBMCs培养上清液的细胞因子IL-12,IFN-r,IL-4和IL-6水平。多组均数间比较采用单因素方差分析。结果:符合入选条件中重度LN患者10例和健康对照9例,全部女性。与健康对照组相比,不论有无PHA刺激,LN患者PBMCs分泌Th1型细胞因子(IL-12,IFN-r)和Th2型细胞因子(IL-4,IL-6)水平均明显升高;与无A771726处理培养的LN患者PBMCs相比,不论有无PHA刺激,经A771726处理培养LN患者PBMCs分泌IL-12、IFN-r、IL-6水平均显著降低,但IL-4水平无明显改变。结论:LEF可明显下调LN患者PBMCs分泌IL-12、IFN-r、IL-6水平可能是其有效治疗LN的又一重要机制。Objective To investigate the effects of leflunomide on the levels of IL-12, IFN-γ, IL-4, and IL-6 secreted by the peripheral blood mononuclear cells （PBMCs） in patients with lupus nephritis （LN）. Methods PBMCs separated from both moderate or severe active LN patients （n = 10, female） and healthy controls （n = 9, female） were cultured with blank, phytohaemagglutinin （PHA） and/or leflunomide active metablite （A771726）. ELISA method was utilized to measure the levels of IFN-γ, IL-12, IL-4, and IL-6 in the supernatants of cultured PBMCs from LN patients. Results Whether treated with PHA or not, the levels of IL-12, IFN-γ, IL-4, and IL-6 were all significantly elevated in the supernatants of cultured PBMCs from LN patients, as compared with healthy controls. And the levels of IFN-γ,IL-12, and IL-6 secreted by PBMCs from LN patients was significantly reduced by A771726, while the level of IL-4 was not significantly changed, as compared with PBMCs from LN patients hadn′t treated with A771726. Conclusions It may be another important mechanism to effectively treat LN that leflunomide can significantly down-regulate the productions of IL-12, IFN-γ and IL-6 by PBMCs from moderate or severe active LN patients.

关 键 词：狼疮肾炎 细胞因子 来氟米特 

分 类 号：R593.242[医药卫生—内科学]